High Prevalence of Bovine Viral Diarrhea Virus-1
in Sheep Abortion Samples
with Pestivirus Infection in Turkey
Hakan Bulut1*,
Ibrahim Sozdutmaz2, Zuleyha Pestil3, Hasan
Abayli1, Ahmet Sait3 and Aydin Cevik4
1Department of Virology, Faculty of Veterinary
Medicine, Firat University, Elazig, Turkey; 2Department
of Virology, Faculty of Veterinary Medicine, Erciyes University,
Kayseri, Turkey; 3Pendik Veterinary Control Institute,
Istanbul, Turkey; 4Department of Pathology, Faculty of
Veterinary Medicine, Firat University, Elazig, Turkey *Corresponding author:hbulut1@firat.edu.tr
Abstract
The aim of this study is
the detection of
prevalenceof bovine viral diarrhea virus-1 (BVDV-1) in sheep abortion samples in
Turkey. For this purpose, initially, the detection of pestiviruses, bluetongue virus (BTV)
and akabane virus (AKAV) was performed by reverse transcription-polymerase chain reaction (RT-PCR) from the aborted sheep fetus specimens.
Then, for differentiation of pestiviruses, further investigations were carried
out from the pestivirus positive samples. Pestiviruses RNAs were detected from
98 (24.74%) of 396 aborted fetus samples collected from 13 provinces in Turkey.
By using border disease virus (BDV) and bovine viral diarrhea virus (BVDV) specific primers, 58 (59.18%) of pestivirus
positive 98 samples were detected as BDV and the remaining 40 (40.81%) were found as BVDV with
RT-PCR. According to the results, BVDV
RNAs were detected from 10.10% (40/396) of aborted fetus specimens. By sequencing and phylogenetic analysis of BVDV
positive four samples, BVDV-1 was identified as the genotype of BVDV. The BTV and AKAV genome were not detected from the
396 aborted samples tested in this study. In conclusion, there's a high ratio of
BVDV-1 among sheep abortions in Turkey. According to this study results, we
think that the high prevalence of BVDV in sheep abortion cases may be important for the control
programme against persistent BVDV infections.
To Cite This Article: Bulut H, Sozdutmaz I, Pestil Z, Abayli H, Sait A and
Cevik A, 2018. High prevalence of bovine viral diarrhea virus-1 in
sheep abortion samples with pestivirus infection in turkey.
Pak Vet J, 38(1): 71-75.
http://dx.doi.org/10.29261/pakvetj/2018.014