Tibial dyschondroplasia (TD) is a skeletal abnormality in fast growing bird, which causes down grading and serious poultry welfare issues in meat industry. TD is described as a non-viable and un-mineralized cartilage in the tibial growth plate of chickens which fails to become bone. The present study was designated to check the effect of Tetramethylpyrazine (TMP) on liver oxidative stress and toxicity in thiram induced tibial dyschondroplasia chickens. One hundred and eighty (1-day old) broiler chickens were equally divided into three groups including Control, Thiram induced (50mg/kg/d) and TMP treatment (30 mg/kg/d). All groups were raised under standard hygienic conditions and chickens were slaughtered to collect blood, liver and tibial bone samples on day 7, 10, 14 and 18 from each group. Thiram caused lameness, and significantly decreased ALP activity and increased ALT and AST contents in the serum of thiram group as compared to control group. The level of GSH-Px, T-AOC and SOD was significantly decreased (P<0.05), while the MDA value and size of growth plate (GP) was increased in TD afflicted chickens as compared to control group. However, the TMP administration increased the ALP contents, and decreased the AST and ALT contents significantly (P<0.05). Furthermore, it increased SOD, T-AOC and GSH-Px and decreased MDA contents significantly (P<0.05). TMP treatment to TD afflicted birds prevented lameness, while reinstated the size of growth plate, angiogenesis, and antioxidant imbalance. TMP may be effective to treat and control of TD along with minimizing the liver damages in TD chickens.