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Milk Indirect-ELISA and Milk Ring Test for Screening of Brucellosis in Buffaloes, Goats and Bulk Tank Milk Samples Collected from Two Districts of Punjab, Pakistan
Tayyaba Israr Khan1, Syed Ehtisham-ul-Haque1*, Usman Waheed1, Iahtasham Khan2, Muhammad Younus3 and Shahzad Ali4
1Department of Pathobiology (Microbiology section); 2Department of Clinical Sciences (Epidemiology and Public Health section); 3Department of Pathobiology (Pathology section), College of Veterinary and Animal Sciences, Jhang, Pakistan; 4Department of Wild Life and Ecology (Zoological Section), University of Veterinary and Animal Sciences (Ravi Campus) Pattoki; *Corresponding author:


Bovine and caprine brucellosis caused by Brucella abortus and Brucella melitensis, are serious zoonotic problem. In this study, the prevalence and risk factors of brucellosis were studied at herd level in two Districts of Punjab. A total of 300 milk samples from buffaloes, goats and bulk tank milk (BTM) were analyzed through milk ring test (MRT), and through a commercially available Milk Indirect ELISA (i-ELISA) kit detecting both B. abortus and B. melitensis antibodies. Higher prevalence was found in goats (76%) followed by BTM samples (42%) and buffalo samples (15%) as tested though Milk i-ELISA. Diagnostic sensitivity (DSe) and specificity (DSp) of MRT was evaluated considering Milk i-ELISA as gold standard test for diagnosis of B. abortus and B. melitensis infection. DSe and DSp of MRT for buffalo milk samples was found as 78.9% and 100% respectively. While for goat samples DSe and DSp of MRT was found as 51.7% and 100% respectively. It is concluded that, MRT is less effective for diagnosis of Brucella antibodies in goat milk as compared to buffalo. Adult age, self-reared animals and larger herd size were found as potential risk factors for the spread of brucellosis in both goat and buffalo. Whereas, use of natural insemination method was found as a risk factor in goat population only and artificial insemination in buffalo only. With the advent of corporate dairy sector in the Pakistan, it is highly recommended that routine Brucella screening should be done using Milk i-ELISA on pooled milk samples.

To Cite This Article: Khan TI, Ehtisham-ul-Haque S, Waheed U, Khan I, Younus M and Ali S, 2018. Milk indirect-ELISA and milk ring test for screening of brucellosis in buffaloes, goats and bulk tank milk samples collected from two districts of Punjab, Pakistan. Pak Vet J, 38(1): 105-108.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)