This study was conducted to determine the concentrations of various trace elements in thiram induced tibial dyschondroplasia chickens. A total 30 day-old male chicks equally divided into two groups including thiram group (n=15) and control group (n=15). Control group was given basal diet throughout the study. Tibial dyschondroplasia was induced by adding thiram @ 50 mg/kg into basal diet from day 3 post-hatch till the end of experiment. Two milliliter bloods were collected from ten chickens of each group on day 7 and 14. The serum was separated by centrifugation and blood serum was assessed in triplicates by Agilent 7700x equipped with an ASX 250 auto sampler. The results were analyzed by student’s t-test, which revealed that the concentration of Cr, Mn, Fe, Co, Ni, Mo, Cd and Pb in thiram group were increased significantly (P<0.05), while the concentration of Cu in was significantly decreased on day 10 compared to control group. The concentrations of Cr, Mn, Fe, Ni and Pb in thiram groups on day 14 were significantly lower than control group, while concentration of Mo was significantly higher in thiram group. The concentrations of Co, Cu and Cd on day 14 were non-significantly different in both groups. The current study shows that thiram effect Cr, Mn, Fe, Co, Ni, Mo, Cd, Pb and Cu in the chick body significantly which is not a good for chicken health. So, it provides considerations to take the effective measures for poultry feed supplement to prevent the occurrence of TD.