Evaluation of Histopathological and Immunohistochemical
Effects of Metformin HCl-Loaded Beads Formulations in
Streptozotocin-Nicotinamide Induced Diabetic Rats
Behzad Mokhtare1*,
Meltem Cetin2 and Yavuz Selim Saglam3
1
Institute of Health Science, Ataturk University;
2 Department of Pharmaceutical Technology, Faculty of
Pharmacy, Ataturk University; 3 Department of Pathology,
Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum,
Turkey *Corresponding author:
yssaglam1@hotmail.com; yssaglam@atauni.edu.tr
Abstract
Metformin hydrochloride (MHCl) widely used for the
treatment of Type 2 DM. The aim of this study was to investigate the early stage histopathological
and immunohistochemical effects of MHCl and MHCl-loaded Alginate (AL) and AL-Chitosan
(CS) beads on the pancreas of Streptozotocin (STZ)-Nicotinamide (NA) induced diabetic rats.
The rats in the following groupswere sacrificed 72 hours after drug
administration and their pancreatic tissues were removed. The healthy rats
(Control, Group I), diabetic rats (Group II; the fasting blood glucose level higher than 126
mg/dl), diabetic rats
treated with pure drugs (Group III), blank beads (Group IV), MHCl-loaded-AL-CS
(Group V) and AL (Group VI) beads. The
prepared samples of pancreatic tissues were examined by histopathological and
immunohistochemical methods. Severity of the
atrophic appearance of langerhans islet in Groups II, III and IV was high.
The degree of necrotic and
degenerative changes of islets of
langerhans in the Groups V and VI were reduced
compared to the changes in the other groups (Groups II, III and IV; P<0.05). As
immunohistochemical, the shrunken and distorted islet of langerhans with marked
loss immunopositive b cells were observed
for Groups II, III and IV. However, there were improvements in the number of
immunopositive b cells and the diameter of islet of langerhans of
Groups V and VI (P<0.05). MHCl-loaded AL-CS and AL beads might be beneficial
to reduce the histopathological changes of pancreas and thereby, the
effects of diabetes.
To Cite This Article:
Mokhtare B, Cetin M
and Saglam YS, 2018. Evaluation of histopathological and immunohistochemical
effects of Metformin HCl-loaded beads formulations in Streptozotocin-Nicotinamide induced diabetic rats. Pak Vet J, 38(2): 127-132. http://dx.doi.org/10.29261/pakvetj/2018.026