Isolation and Virulence Genes Characterization of
Diarrheagenic Escherichia coli
from Calves
Aneela Pervez1, Faisal Rasheed Anjum1,
Aqsa Asharf Bukhari2, Sidra Anam1,
Sajjad-ur-Rahman1 and Muhammad Imran Arshad1
1Institute
of Microbiology, University of Agriculture, Faisalabad 38000,
Pakistan; 2Department of Microbiology, Institute of Pure
and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan
*Corresponding author:
drimranarshad@yahoo.com
Abstract
Calf diarrhea due to
Escherichia coli (E. coli) causes huge economic losses and possess
an important veterinary health aspect. In current study, antibiotic profiling,
molecular screening, and identification of
stx-1, eaeA, escV and
bfpA virulence genes of E.
coli isolated from calves were carried out. Fecal samples from 28
diarrheagenic calves were taken, processed and cultured on MacConkey’s agar,
followed by identification using biochemical tests. Antibiotic susceptibility of
the isolates was checked by disc diffusion method. For molecular
characterization, polymerase chain reaction (PCR) was performed by using
specific primers. Out of 28 samples collected from diarrheagenic calves, 83%
samples were found positive for E. coli
on the basis of biochemical profiling. Antimicrobial susceptibility against
various antibiotics exhibited that most of the
E. coli isolates were multi-drug resistant. All the isolates showed
100% resistance to rifampicin, erythromycin and oxacillin. The
E. coli isolates were not resistant to
gentamycin. Analysis through PCR showed that 10% of the isolates were positive
for stx-1 gene, however,
bfpA, escV and
eaeA genes were not detected. Thus,
E. coli is one of the key bacterium causing calve diarrhea and it
was found that among all the virulent genes the
stx-1plays a part in E. coli
associated diarrheal infection in calves.
To Cite This Article: Pervez A, Anjum FR, Bukhari AA, Anam S, Rahman SU
and Arshad MI, 2018. Isolation and virulence genes characterization of
diarrheagenic Escherichia coli from
calves. Pak Vet J, 38(2): 133-136. http://dx.doi.org/10.29261/pakvetj/2018.042