Effect of Stabilizers on Infectivity Titer of Freeze Dried Peste Des
Petits Ruminants Virus Vaccine
Muhammad Zubair Latif1, Khushi
Muhammad1, Riaz Hussain2*, Faisal Siddique1, Imran
Altaf1, Muhammad Anees3, Muhammad Imran4,
Muddassar Hameed1 and Muhammad Farooq1
1Department
of Microbiology, University of Veterinary and Animal Sciences
(UVAS), Lahore, Pakistan; 2University College of
Veterinary and Animal Science, The Islamia University of Bahawalpur,
Pakistan; 3Veterinary Research Institute, Lahore,
Pakistan; 4Institute of Biochemistry and Biotechnology
UVAS, Lahore, Pakistan *Corresponding author:
driazhussain@yahoo.com
Abstract
Peste des Petits Ruminants (PPR) is an acute and
highly contagious viral disease of small ruminants caused by Morbilivirus. PPR
induces high morbidity and mortality in infected animals and is responsible for
huge economic losses to livestock holders and dairy industry. Live attenuated
freeze-dried vaccines are commonly used to control the infectious diseases.
There is loss of virus titer during freeze drying and post reconstitution of the
vaccine resulting in the decrease efficacy of vaccine. Stabilizers are therefore
added to protect the virus from freeze drying stress and heat shock during
storage and transportation. Therefore, in present study different stabilizers (Weybridge
medium-WBM, Lactalbumin hydrolysate sucrose-LS, Lactalbumin hydrolysate
sorbitol-LSbG, TrisTrehalose-TT, Tris sucrose-TS and Goat skimmed milk-GSM) were
evaluated to protect the infectivity titer of the virus. The stabilizers having
carbohydrates (sucrose, sorbitol and trehalose), salts (sodium glutamate) and
hydrolyzed proteins (Lactalbumin hydrolysate) are effective to make compact mass
of PPR virus vaccine. Casein or other proteins (not hydrolyzed) in stabilizers
may act as allergens and could cause anaphylactic shock in the vaccinated
animals.However, TS did not make compact
mass (cake) of vaccine but all the contents of the virus suspension evaporated
while WBM, LS, GSM and LSbG maintained infectivity titer of the vaccine virus
(if reconstituted with PBS and stored at 4°C) for 12 hours. However, Tris
Trehalose-TT is able to protect infectivity titer of the vaccine virus during
freeze drying and even during its storage after rehydration with PBS at 4°C for
24 hours.
To Cite This Article:
Latif MZ, Muhammad K, Hussain R, Siddique F, Altaf
I, Anees M, Imran M, Hameed M and Farooq M, 2018.
Effect of stabilizers on infectivity
titer of freeze dried Peste des Petits Ruminants virus vaccine. Pak Vet
J, 38(2): 169-173. http://dx.doi.org/10.29261/pakvetj/2018.032