The emergence of variant pseudorabies virus caused great economic losses in domestic farms in the recent past. The mice were infected with Halv strain PRV via intragastric administration, intranasal and subcutaneous into the cervical skin to study the Pseudorabies in mouse model. The gross lesions, microscopic lesions and the distribution of PRV in mice were investigated. All the mice in each group showed exciting and roughening of the fur and frequent scratching. Microscopic lesions included mild-to-severe interstitial pneumonia, hemorrhage and nonsuppurative encephalitis in brain. The distribution of PRV in three groups was different. In intragastric administration group, PRV antigens were distributed in brain hippocampus, medulla oblongata, gray substance spinal cord and intestinal submucosa. In intranasal group, PRV antigens were detected in gray substance spinal cord, pons, myocardial cells, pulmonary epithelial cells and lymphocytes in red pulp of the spleen. In subcutaneous group, the PRV antigens were located in the sensory and motor cortex, prefrontal cortex, gray matter in spinal cord, purkinje cells in the cerebellum and lymphocytes in the splenic red pulp. These findings provided evidence that the distribution of PRV was depended on injected-routes.

To Cite This Article: Fu ZQ, Li YC, Liu XL, Gu CQ, Hu XY, Cheng GF and Zhang WP, 2018. Distribution of pseudorabies virus in kunming strain mouse via gavage, intranasal and subcutaneous infection. Pak Vet J, 38(2): 174-178. http://dx.doi.org/10.29261/pakvetj/2018.029