1Obstetrics Gynecology and
Animal Reproduction Clinic; 2Pre-Clinical Veterinary
Science Department, Faculty of Veterinary Medicine, Mahanakorn
University of Technology, Bangkok, 10530, Thailand; 3Department
of Obstetrics Gynecology and Reproduction, Faculty of Veterinary
Science, Chulalongkorn University, Bangkok, 10330, Thailand *Corresponding author:
siriwat.s@chula.ac.th
Abstract
The relationship between kisspeptin and GnRH
releasing in many species has been studied including post-pubertal buffalo, but
not in pre-pubertal buffalo. The aims of this study were to detect the
localization of Kiss1mRNA and the distribution of kisspeptin protein in
the preoptic (POA) and arcuate (ARC) hypothalamic nuclei of pre-pubertal swamp
buffaloes. Brains were collected from ten pre-pubertal female buffaloes (<1
year, 1-1.5 years, 1.5-2 years, 2-2.5 years and 2.5-3 years; two animals each
age) and processed for paraffin blocks. Four-micron paraffin sections of the POA
and ARC hypothalamic nuclei were prepared. The present research found evidence
of Kiss1 mRNA in the cytoplasm of the neuronal soma and some small
neuronal cells using the in-situ hybridization technique in all ten heifers.
Using the immunohistochemistry technique, kisspeptin proteins expressed a weak
intensity in the cellular process of neurons. The distribution of kisspeptin
immunoreactions were found mainly in the POA hypothalamic nucleus in the
juvenile group (<1 years) and only in the ARC area in the 1-2.5 years old
pre-pubertal group. However, there was no kisspeptin reaction in both
hypothalamic nuclei in the 2.5-3 years (peri-pubertal) group (P<0.01). This
study provides evidence of Kiss1 mRNA and kisspeptin protein in the
hypothalamus of pre-pubertal buffaloes. This suggests that kisspeptin may be
involved in reproductive development and may influence puberty onset in swamp
buffalo heifers.
To Cite This Article: Chaikhun-Marcou T,
Sotthibandhu P, Yanprapasiri C,
Pavasutthipaisit S and Suadsong S,
2018. Kiss1 mRNA and its protein distribution in preoptic and arcuate hypothalamic
nuclei in pre-pubertal female swamp buffaloes. Pak Vet J, 38(2): 137-142.
http://dx.doi.org/10.29261/pakvetj/2018.033