previous page   Pak Vet J, 2018, 38(2): 184-188   next page
Genotype Characterization of Newcastle Disease Virus Isolated from Commercial Chicken Flocks in West Java, Indonesia
Dwi Desmiyeni Putri1,2*, Ekowati Handharyani3, Retno Damajanti Soejoedono4, Agus Setiyono3, Ni Luh Putu Ika Mayasari4 and Okti Nadia Poetri4
2Study Program of Animal Husbandry, Department of Animal Husbandry, State Polytechnic of Lampung, Lampung, Indonesia; 3Department of Veterinary Clinic Reproduction and Pathology; 4Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia
*Corresponding author:;


Newcastle disease (ND) is a worldwide very contagious poultry disease, caused by Newcastle disease virus (NDV). Despite the vaccination, ND outbreaks in Indonesia’s commercial chicken flocks have been reported regularly.  Our study aimed to determine the genotype of isolates and genetic relatedness with other Indonesia’s NDVs published on the GenBank. Four NDV isolates were obtained from vaccinated flocks in 2011, 2014, 2015 in West Java, Indonesia. Two NDVs belong to virulent strain and the other two belong to avirulent strain.  Phylogenetic analyses of F gene revealed that NDV/Ck/BGR/11 and NDV/Ck/GS/14 belong to genotype VII sub-genotype (h) and (i); whilst NDV/Ck/CJR/15 and NDV/Ck/BGR/15 belong to genotype II.  The virulent NDVs were clustered in the same genotype and closely related to earlier Indonesia’s NDVs isolated in 2007, 2009 and 2010.  Result of current study showed that recent NDVs of sub-genotype VIIh and VIIi circulating in commercial chicken farm in West Java, Indonesia have high similarity with NDVs isolated during 2007 and 2010 in Indonesia. Our findings may be valuable for future studies to develop improved control and diagnostic strategies of ND.

To Cite This Article: Putri DD, Handharyani E, Soejoedono RD, Setiyono A, Mayasari NI and Poetri ON, 2018. Genotype characterization of Newcastle disease virus isolated from commercial chicken flocks in West Java, Indonesia. Pak Vet J, 38(2): 184-188.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)