Genotype Characterization of Newcastle Disease Virus Isolated from
Commercial Chicken Flocksin West Java, Indonesia
Dwi Desmiyeni Putri1,2*, Ekowati
Handharyani3, Retno Damajanti Soejoedono4,
Agus Setiyono3, Ni Luh Putu Ika Mayasari4and Okti
Nadia Poetri4
2Study
Program of Animal Husbandry, Department of Animal Husbandry, State
Polytechnic of Lampung, Lampung, Indonesia; 3Department
of Veterinary Clinic Reproduction and Pathology; 4Department
of Animal Diseases and Veterinary Public Health, Faculty of
Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia
*Corresponding author:
ekowatieko@yahoo.com; desmiyenidwi@gmail.com
Abstract
Newcastle disease (ND) is a worldwide very
contagious poultry disease, caused by Newcastle disease virus (NDV). Despite the
vaccination, ND outbreaks in Indonesia’s commercial chicken flocks have been
reported regularly.Our study aimed
to determine the genotype of isolates and genetic
relatedness with other Indonesia’sNDVs published on the
GenBank. Four NDV isolates were obtained from vaccinated
flocks in 2011, 2014, 2015 in West Java, Indonesia. Two NDVs belong to virulent strain and the other two belong to
avirulent strain.Phylogenetic analyses of F gene
revealed that NDV/Ck/BGR/11 and
NDV/Ck/GS/14 belong to genotype VII sub-genotype (h) and (i);whilst NDV/Ck/CJR/15 and NDV/Ck/BGR/15 belong to genotype II.The virulent NDVs were clustered in the same genotype and closely
related to earlier Indonesia’s NDVs isolated in
2007, 2009 and 2010.Result of
current study showed that recent NDVs of sub-genotype VIIh and VIIi circulating
in commercial chicken farm in West Java, Indonesia have high similarity with
NDVs isolated during 2007 and 2010 in Indonesia. Our findings may be valuable
for future studies to develop improved control and diagnostic strategies of ND.
To Cite This Article: Putri DD, Handharyani E, Soejoedono RD, Setiyono A, Mayasari NI and Poetri ON, 2018.
Genotype characterization of Newcastle disease virus isolated from commercial
chicken flocksin West Java, Indonesia. Pak Vet J, 38(2): 184-188.
http://dx.doi.org/10.29261/pakvetj/2018.041