Isolation and Molecular Characterization of Bovine
Trichophyton verrucosum
Strains Based on Sequence Analysis of Internal Transcribed Spacer
Region (ITS1) and Microsatellite Loci
Osman Yaşar Tel1, Faruk Bozkaya2*,
Akın Yiğin2, Sevil Erdenliğ Gürbilek1 and
Oktay Keskin1
1Department
of Microbiology, Faculty of Veterinary Medicine, Harran
University-63300 Sanliurfa, Turkey
2Department of Genetics, Faculty of Veterinary
Medicine, Harran University-63300 Sanliurfa, Turkey
*Corresponding author:
farukbozkaya@yahoo.com
Abstract
The aim of this study was to isolate
Trichophyton verrucosum from cattle
and investigate the genetic diversity of the isolated strains by sequencing ITS1
region and by fragment length analysis of microsatellite loci. In this study, 84
hair and skin scraping samples from cattle with dermatophytosis were
investigated by direct microscopy and culture methods. Furthermore, DNA was
isolated from the colonies and sequence of ITS1 region was determined by direct
sequencing. In addition, primers were designed for amplification
of 11 loci showing simple tandem repeats (CHFD, SRT, HP1, HP2, HP3, HP4 TVMS1, TVMS2, TVMS3, TVMS4 and TVMS5)and PCR products of each
locus were examined by fragment length analysis. Out
of 84 samples 25 (29.7%) T. verrucosum
strains were isolated. Sequence analysis of ITS1 region revealed that all strains have the same sequence. The observed fragment lengths of the microsatellite loci CHFD, SRT, HP1, HP2, HP3, HP4 TVMS1, TVMS2, TVMS3,
TVMS4 and TVMS5 were 136 bp, 170 bp 178 bp, 423 bp, 356 bp, 225 bp, 232 bp, 201
bp, 270bp, 173 bp, respectively. None
of the microsatellite loci examined showed fragment length variation among the
samples included into the study. The results suggested
that dermatophyte species could be distinguished based on the sequence of ITS1 region, while absence of sequence variation at
ITS1 region did not allow distinguishing between
T. verrucosum strains. On the other hand, no
fragment length variation of the microsatellite loci among the T. verrucosum strains examined was
observed.
To Cite This Article: Tel OY, Bozkaya F, Yiğin A, Gürbilek SE and
Keskin O, 2018. Isolation and molecular
characterization of bovine Trichophyton
verrucosum strains based on sequence analysis of Internal Transcribed Spacer
Region (ITS1) and microsatellite Loci. Pak Vet J, 38(2): 189-193. http://dx.doi.org/10.29261/pakvetj/2018.040