Estimation of Florfenicol Residues in Layer Meat
and Egg Samples using High Performance Liquid Chromatography
Amna Fahim1, Bilal
Aslam1*, Mashkoor Mohsin2, Ahmad Raza1,
Muhammad Naeem Faisal1 and Asif Hussain1
1Institute
of Pharmacy, Physiology and Pharmacology, University of Agriculture,
Faisalabad-38040, Pakistan 2Institute of Microbiology,
University of Agriculture, Faisalabad-38040, Pakistan *Corresponding author: cba933@gmail.com
Abstract
Antibiotics are widely used in the poultry
industry to enhance the health and productivity of flocks which may have adverse
effects on consumer’s health. It is necessary to screen food products from
animal origin for antimicrobial residues to safeguard the consumer’s health. The
present study was aimed to detect florfenicol (FF) residues in meat and egg
samples of layer birds. For this purpose 150 meat and eggs samples were
collected in equal ratio. High performance liquid chromatography (HPLC) was used
to determine residual concentration of FF in meat and egg samples at wavelength
of 223 nm. Ethyl acetate and phosphate buffer saline solution were used for
extraction of FF from the samples. The mobile phase contained acetonitrile and
water (27:73 v/v). Mean residual concentrations of FF as 61.56±13.19 and
281.08±57.46 µg/kg in meat and egg samples was detected. This study also showed
that 80% (60) meat and 72% (54) egg samples were FF residue positive, out of
these 86.7% (52) meat and 55.6% (30) egg samples were found to have residual
concentrations above maximum residual limits. This contaminated meat may cause
public health issues. There is a need to develop legislation about residual
concentration of drugs in animal food products in Pakistan as well as to inform
formers about the detrimental effects of drug residues on human health.
To Cite This Article: Fahim A, Aslam B, Mohsin M, RazaA,
Faisal MN and Hussain A, 2018. Estimation of florfenicol residues in layer meat
and egg samples using high performance liquid chromatography. Pak Vet J, 38(3):
329-332. http://dx.doi.org/10.29261/pakvetj/2018.052