PAKISTAN
VETERINARY
JOURNAL
     
 
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Effect of Melatonin on Casein Expression in Buffalo Mammary Epithelial Cells
 
Hasan Riaz12, Ting-Xian Deng1, Xiao-Ya Ma1, Sha-Sha Liang1, An-Qin Duan1, Xing-Rong Lu1, Muhammad Rizwan Yousuf3, Chun-Ying Pang1 and Xian-Wei Liang1*
 
1Key Laboratory of Buffalo Genetics, Breeding and Reproduction, Ministry of Agriculture, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, Guangxi, P. R. China; 2Department of Biosciences, COMSATS University Islamabad, Sahiwal Campus, Pakistan; 3Department of Theriogenology, University of Veterinary and Animal Science, Lahore, Pakistan; *Corresponding author: liangbri@126.com
 

Abstract   

The objectives of this study are to find out the effect of melatonin treatments (43µM & 430µM) on mRNA and protein expression of casein, and melatonin receptors in buffalo mammary epithelial cells (BuMEC) using reverse transcriptional polymerase chain reaction (qRT-PCR) and Western blotting. Furthermore, by using specific inhibitors against mTOR (Rapamycin) and Jak2-Stat5 pathway (AG490), effect of melatonin treatment on translational level of casein was measured. The results showed that melatonin treatment (430 µM) significantly up-regulated casein level both at transcriptional (P<0.001) and translational (P<0.05) levels. Melatonin treatment significantly up-regulated mRNA expression of (P<0.05) melatonin receptor 1a (MT1) but melatonin receptor 1b (MT2) did not significantly varied. In the presence of mTOR inhibitor, rapamycin, melatonin failed to up regulate (P<0.05) protein expression of casein but no significant difference was found in the level of casein in the presence of Jak2-Stat5 inhibitor (AG490). These results indicate the significance of melatonin in altering milk casein expression in mammary epithelial cells in buffalo. Keeping in view the seasonality of buffalo in tropical and subtropical region, this study is important in the establishment of melatonin as the key modulator in changing concentration of casein in milk.

To Cite This Article: Riaz H, Deng TX, Ma XY, Liang SS, Duan AQ, Lu XR, Yousuf MR, Pang CY and Liang XW, 2018. Effect of melatonin on casein expression in buffalo mammary epithelial cells. Pak Vet J, 38(3): 333-336. http://dx.doi.org/10.29261/pakvetj/2018.065  

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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