Effect of Melatonin on Casein Expression in
Buffalo Mammary Epithelial Cells
Hasan Riaz12, Ting-Xian Deng1,
Xiao-Ya Ma1, Sha-Sha Liang1, An-Qin Duan1,
Xing-Rong Lu1, Muhammad Rizwan Yousuf3,
Chun-Ying Pang1 and Xian-Wei Liang1*
1Key
Laboratory of Buffalo Genetics, Breeding and Reproduction, Ministry
of Agriculture, Buffalo Research Institute, Chinese Academy of
Agricultural Sciences, Nanning, Guangxi, P. R. China; 2Department of Biosciences, COMSATS
University Islamabad, Sahiwal Campus, Pakistan; 3Department of Theriogenology,
University of Veterinary and Animal Science, Lahore, Pakistan;
*Corresponding author: liangbri@126.com
Abstract
The objectives of this study are to find out the
effect of melatonin treatments (43µM & 430µM) on mRNA and protein expression of
casein, and melatonin receptors in buffalo mammary epithelial cells (BuMEC)
using reverse transcriptional polymerase chain reaction (qRT-PCR) and Western
blotting. Furthermore, by using specific inhibitors against mTOR (Rapamycin) and
Jak2-Stat5 pathway (AG490), effect of melatonin treatment on translational level
of casein was measured. The results showed that melatonin treatment (430 µM)
significantly up-regulated casein level both at transcriptional (P<0.001) and
translational (P<0.05) levels. Melatonin treatment significantly up-regulated
mRNA expression of (P<0.05) melatonin receptor 1a (MT1) but melatonin receptor
1b (MT2) did not significantly varied. In the presence of mTOR inhibitor,
rapamycin, melatonin failed to up regulate (P<0.05) protein expression of casein
but no significant difference was found in the level of casein in the presence
of Jak2-Stat5 inhibitor (AG490). These results indicate the significance of
melatonin in altering milk casein expression in mammary epithelial cells in
buffalo. Keeping in view the seasonality of buffalo in tropical and subtropical
region, this study is important in the establishment of melatonin as the key
modulator in changing concentration of casein in milk.
To Cite This Article:
Riaz H, Deng TX, Ma XY, Liang SS, Duan AQ, Lu XR,
Yousuf MR, Pang CY and Liang XW, 2018. Effect of melatonin on casein expression
in buffalo mammary epithelial cells. Pak Vet J, 38(3): 333-336. http://dx.doi.org/10.29261/pakvetj/2018.065