Antibacterial Effect of Different Concentrations
of Silver Nanoparticles
A. Ebru Borum1* and Ertan Güneş2
1Department
of Microbiology, Faculty of Veterinary Medicine, Balıkesir
University, Balıkesir, Turkey 2Vocational School of Technical
Sciences, Uludag University, Bursa, Turkey *Corresponding author:
ebruborum@balikesir.edu.tr
Abstract
Silver has been in use since time immemorial in
the form of metallic silver, silver nitrate, silver sulfadiazine for the
treatment of burns, wounds and several bacterial infections. Silver has long been known to show a strong antimicrobial effect to
microorganisms. The antimicrobial effect of 30 and 100 ppm
silver nanoparticles were investigated against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium,
Enterococcus faecalis, Bacillus cereus, Bacillus subtilis, Paenibacillus larvae,
Candida albicans andAspergillus niger.
The microorganisms were diluted with sterile distilled water and prepared
dilutions of 106 of test microorganisms. Dilutions of microorganisms
cultured to blood agar base and incubated at 37°C
for 24 hours. One mL dilution of
106
of all of microorganisms was centrifuged at 3500 rpm for 20 minutes then
30 and 100 ppm of silver nanoparticle solutions were added. Samples were
inoculated in blood agar for different time intervals
i.e., 0, 2, 5, 10, 30, 60 minutes and 24 hour. Antibacterial activity of silver nanoparticles against various
microorganisms was detected at 0, 2, 5, 10, 30, 60 minutes and 24 hours. As
results, yeast, fungi and bacteria were inhibited at 30 and 100 ppm. But,
P. larvae were not inhibited, while
B. subtilis also could not be
inhibited at 30 ppm. The antibacterial activity of 100 ppm was
stronger than the antibacterial activity of 30 ppm of nanoparticles. Nanosilver
is very effective to important pathogens.