Screening, Characterization and Physicochemical
Optimization of Phosphorus Solubilization Activity of Potential
Probiotic Lactobacillusspp.
Aanisa Arif1, Muhammad Nawaz1*,
Masood Rabbani1, Sanaullah Iqbal2, Amina
Mustafa1, Muhammad Rizwan Yousuf3 and Khushi
Muhammad1
1Department
of Microbiology; 2Department
of Food Sciences and Human Nutrition; 3Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore,
Pakistan *Corresponding author:muhammad.nawaz@uvas.edu.pk
Abstract
Present study was designed to develop indigenous
phytate hydrolyzing probiotic
Lactobacilli to increase phosphorous availability in poultry gut with ultimate
goal of efficient and enhanced poultry production. A total of 90 Lactobacilli
were isolated from indigenous poultry droppings and homemade fermented milk
products. Lactobacilli were screened for phytate hydrolyzing ability and enzyme
activities were measured calorimetrically by ammonium molybdate assay. Out of 62
isolates showing phytate hydrolysis, 16 were confirmed by cobalt chloride
staining. Out of 16, three isolates (PDP10, PDP24 and FYP38) were identified
as Lactobacillus gallinarum,
Lactobacillus reutri and
Lactobacillus fermentum respectively,
and further selected for physico-chemical optimization of phytase production.
PDP10, PDP24 and FYP38 showed highest phytase activity at 35°C (6.86±0.15,
5.12±0.12 and 5.65±0.13 IU/ml,
respectively), pH 5.0 (6.86±0.15,
5.12±0.11 and 5.50±0.13 IU/ml
respectively), 1% NaCl (4.78±0.14,
4.18±0.13 and 5.58±0.02 IU/ml
respectively) and 0.3% bile salts (3.76±0.12, 1.12±0.11 and 2.21±0.15
IU/ml respectively). Glucose was optimum carbon source for phytate hydrolysis.
Optimum nitrogen source was peptone for PDP10 and PDP24 while tryptone for
FYP38. It is concluded that phytate hydrolyzing Lactobacilli reported in this
study might be used as probiotics for increased phosphorous availability in
poultry after further investigations.
To Cite This Article: Arif A, Nawaz M, Rabbani M, Iqbal S, Mustafa A,
Yousuf MR and Muhammad K, 2018. Screening, characterization and
physicochemical optimization of phosphorus solubilization activity of potential
probiotic Lactobacillus spp. Pak Vet
J, 38(3): 316-320. http://dx.doi.org/10.29261/pakvetj/2018.061