Prevalence of
Neospora caninum using Milk and Serum ELISA and its Hematological
Effect in Dairy Buffaloes
Amar Nasir1*, Muhammad Arfan Zaman2,
Abdul Shakoor1, Tauseef-ur-Rehman4, Iahtasham
Khan1, Syed Ehtisham-ul- Haque2, Arbab
Sikandar3, Sabiqaa Masood5 and Hamid Akbar6
1Department
of Clinical Sciences; 2Department of Pathobiology; 3Department
of Basic Sciences, College of Veterinary & Animal Sciences, Jhang
(Sub-campus, University of Veterinary & Animal Sciences, Lahore)
Pakistan 4University College of Veterinary
and Animal Sciences, The Islamia University Bahawalpur-Pakistan 5Department of Parasitology, Faculty
of Veterinary Science, University of Agriculture, Faisalabad,
Pakistan 6Department of Clinical Medicine and
Surgery, University of Veterinary & Animal Sciences, Lahore),
Pakistan *Corresponding author: amar.nasir@uvas.edu.pk
Abstract
The current study compared the performance of
serum and milk ELISA in determining
Neospora caninum prevalence in lactating Nili-Ravi buffaloes. Overall, 64 milk samples (n=57
individual milk samples; n=7 bulk milk samples) collected from buffaloes of
districts Lahore and Narowal were tested for anti-N. caninum antibodies using milk iscom ELISA. The same animals were
evaluated serologically using serum cELISA for this purpose. Furthermore,
hematological profiling of 50 seropositive and 15 seronegative buffaloes was
performed to appraise any effect of N.
caninum seropositivity on the hematological parameters of the host.
Resultantly, milk ELISA detected lesser milk samples positive i.e., 61.64%
(±12.6, 95% C.I. 35/57) for N. caninum
against higher seropositivity (76.6% ±7.16, 42/57) by serum cELISA. The
agreement level (Kappa ratio=0.568 & R2 =0.769) between the
performance of two assays was found good. General Linear Model analysis of
hematological parameters reflected significantly (P<0.05) lower monocyte count but higher blood glucose levels in
seropositive buffaloes associated with different seasons. It was concluded that,
owing to the limitations of the milk ELISA (also lower sensitivity than cELISA)
it is best suited for finding prevalence in lactating dairy herds having
substantial number of infected animals but in general, serum ELISA is best for
prevalence studies. While, decreased monocyte count and higher blood glucose
levels in the affected animals was observed, which may be attributed to
persistent inherent stress of infection on the host.
To Cite This Article: Nasir A, Zaman MA, Shakoor A, Rehman TU, Khan I,
Ehtisham-ul-Haque S, Sikandar A, Masood S and Akbar A, 2018. Prevalence of
Neospora caninum using milk and serum
ELISA and its hematological effect in dairy buffaloes. Pak Vet J, 38(3):
281-285. http://dx.doi.org/10.29261/pakvetj/2018.039