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Detection of Actinobacillus pleuropneumoniae through Duplex PCR Based on ApxIA and ApxIVA Genes
Huayuan Lin1,2§, Huirong Mao1§, Sufang Cheng1§, Mengying Yi2, Yezhao Ruan1, Cong Wu1, Zheng Xu1, Guoliang Hu1, Xiaoquan Guo1, Huabin Cao1, Lin Li1, Yinhua Han3 and Ping Liu*1
1Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, P. R. China; 2National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; 3Guangxi Center for Animal Disease Control and Prevention, Nanning 530001,Guangxi,PR China
*Corresponding author:


Actinobacillus pleuropneumoniae (APP) is the bacterial pathogen of pleuropneumoniae, a severe contagious and fatal respiratory disease of swine. For the purpose of setting up an easy way to detect this bacteria in swine, a duplex polymerase chain reaction (PCR) assay was built up for the detection of APP. Two pairs of primers were designed according to the APP ApxI and ApxIV gene sequences. Using these primers, a duplex PCR method was developed to fast identify APP in different bacteria samples, such as Enterococcus faecalis, Escherichia coli from cattle and swine, Staphylococcus aureus and Streptococcus. Our results have shown that duplex PCR could be a potential molecular method for the diagnosis of APP infection in swine.

To Cite This Article: Lin H, Mao H, Cheng S, Yi M, Ruan Y, Wu C, Xu Z, Hu G, Guo X, Cao H, Li L, Han Y and Liu P, 2018. Detection of Actinobacillus pleuropneumoniae through duplex PCR based on apxia and apxiva genes. Pak Vet J, 38(3): 276-280.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)