Actinobacillus pleuropneumoniae (APP) is the bacterial pathogen of pleuropneumoniae, a severe contagious and fatal respiratory disease of swine. For the purpose of setting up an easy way to detect this bacteria in swine, a duplex polymerase chain reaction (PCR) assay was built up for the detection of APP. Two pairs of primers were designed according to the APP ApxI and ApxIV gene sequences. Using these primers, a duplex PCR method was developed to fast identify APP in different bacteria samples, such as Enterococcus faecalis, Escherichia coli from cattle and swine, Staphylococcus aureus and Streptococcus. Our results have shown that duplex PCR could be a potential molecular method for the diagnosis of APP infection in swine.

To Cite This Article: Lin H, Mao H, Cheng S, Yi M, Ruan Y, Wu C, Xu Z, Hu G, Guo X, Cao H, Li L, Han Y and Liu P, 2018. Detection of Actinobacillus pleuropneumoniae through duplex PCR based on apxia and apxiva genes. Pak Vet J, 38(3): 276-280. http://dx.doi.org/10.29261/pakvetj/2018.058