1Department of Pathology,
University of Veterinary and Animal Sciences, Lahore, Pakistan;
2Poultry Research Institute, Rawalpindi; 3Department
of Zoology, University of the Punjab, Pakistan; 4Center
for Applied Molecular Biology (CAMB), University of the Punjab,
Lahore *Corresponding author:
bz-leo@hotmail.com
Abstract
The Infectious bursal disease virus (IBDV),
rapidly destroy immature B lymphocytes in bursa of fabricous and cause the
immunosuppression and high mortality in broiler chicken. Therefore, present
study was conducted to investigate the viral antigen in lymphoid tissues of
experimentally infected broiler chicken and specific antigen negative (SAN)
chicks through immunohistochemistry (IHC) and reverse transcriptase polymerase
chain reaction (RT-PCR). For this purpose, two hundred broiler chicks were
reared and divided into 4 groups (A, B, C and D), fifty birds in each group. The
birds in group A were commercial broiler chicks having maternal derived
antibodies and group B containing SAN chicks. The groups A and B were challenged
with IBDV field isolate. While the group C and D was unchallenged control. On 3rd,
5th, 9th and 14th day post infection (pi),
Lymphoid organs Bursa of Fabricius, spleen and thymus and non lymphoid organs
kidney and liver were collected for histopathology, IHC and viral genome
detection by RT-PCR. At day 3 of pi, viral antigen observed in lymphoid cells of
follicles of bursa and thymus, marked leukocytic infiltration occurred with
bursal oedema and hyperemia. Virus was constantly found at 5th day
post infection until day 14th, in the primary lymphoid tissues and
characterized in acute inflammation. The hypervariable region of viral antigen,
VP 2 gene was detected in bursa, thymus, spleen, kidney and liver tissues in
challenged groups A and B through RT-PCR by using specific primers of 743bp. The
virus antigen detection from tissues reduced with increasing antibody titer. It
is concluded, the IBDV antigen detection rate in primary lymphoid organs was
high as virus targets the lumphocytes and macrophages. Immunohistochemistry and
RT-PCR are more specific and sensitive methods for detection of IBD viral
antigen.
To Cite This Article: Zahid B, Aslam A, Anjum
R, Imran S,Irshad I, Ara C and
Sadia H, 2018. Study on tissue tropism and molecular detection of VP2 gene of
infectious bursal disease virus in experimentally infected broiler tissues. Pak
Vet J, 38(3): 291-295. http://dx.doi.org/10.29261/pakvetj/2018.054