Effect of Flavonoids
from Eupatorium odoratum
L. on Immunoglobulins and Cecal Microflora in Broilers
Qiao-Hui Zhang1§, Fang-Hong Nie2§,
Xiao-Ning Wang1, Hong-Ying Lin1, Hwa-Chain
Robert Wang3, Ravi Gooneratne4* and Jin-Jun
Chen1*
1Department
of Veterinary Medicine, College of Agricultural Sciences; 2Department
of Food Safety, College of Food Science and Technology, Guangdong
Ocean University, Zhanjiang, Guangdong 524088, China; 3Department
of Biomedical and Diagnostic Sciences, The College of Veterinary
Medicine, University of Tennessee, Knoxville, TN 37996, USA; 4Department
of Wine, Food and Molecular Biosciences, Faculty of Agriculture and
Life Sciences, Lincoln University, Lincoln 7647, New Zealand;
*Corresponding author: jjchen777@aliyun.com (JJC);
Ravi.Gooneratne@lincoln.ac.nz (RG)
Abstract
The effects of total flavonoids from
Eupatorium odoratum L. (TFEO) on
serum (IgM, IgG) and duodenal mucosa (Secretory IgA–SIgA) immunoglobulins and
cecal microflora composition in broiler chicken were investigated. Test
groups received diets supplemented with 100, 200, or 400 mg/kg TFEO for 21 and
42d. At 21d, IgM levels were higher in the 200 and 400 mg/kg groups compared to
control group. IgG and SIgA levels were increased in all test groups (P<0.05).
At 42d, IgG content in all test groups were higher (P<0.05), IgM in all test
groups was higher (P>0.05). SIgA in all test groups increased at 200 and 400
mg/kg (P<0.01) >100 mg/kg (P<0.05) compared to the controls. Significant
increases (P<0.05) in Firmicutes phylum and
Bifidobacterium,
Lactobacillus and
Lachnospira genera gut microbes and significant decreases in
pathogenic Escherichia and
Clostridium of the test groups were
observed at 21 and/or 42d. TFEO flavonoids
are suggested for use as a feed additive for broilers to improve cecal
microflora and humoral immunity.
To Cite This Article:
Zhang QH, Nie FH, Wang XN, Lin HY, Wang HCR,
Gooneratne R and Chen JJ, 2018. Effect of flavonoids from
Eupatorium odoratum L. on
immunoglobulins and cecal microflora in broilers. Pak Vet J, 38(3): 296-300.
http://dx.doi.org/10.29261/pakvetj/2018.068