Molecular and Histopathological Investigation of Avian Infectious
Bronchitis Virus in the Delta of Egypt between 2016 and 2017
Asmaa Magouz1*,
Walied Abdo2, Asmaa Abdelsabour1, Ahmed
Elbestawy3 and Abdelrazik Desouky 4
1Department of
Virology; 2Department of Pathology; 4Department
of Parasitology; Faculty of Veterinary Medicine, Kafrelsheikh
University, Kafrelsheikh, Egypt; 3Department of Poultry
Diseases, Faculty of Veterinary Medicine, Damanhour University,
Damanhour, Egypt *Corresponding author: asmaamagouz@gmail.com
Abstract
Infectious bronchitis virus (IBV) is a highly
contagious viral disease of Family Coronaviridae. The current study reports the
molecular and histopathological investigation of IBV strains emerged in
outbreaks in the Nile Delta of Egypt. Tissue specimens from thirty-six chicken flocks were subjected to virus isolation,
histopathology and PCR.Twenty-three isolates
were confirmed as IBVs based on the amplification of the highly conserved
nucleocapsid (N) gene. Further characterization of five selected isolates was
done by amplification of the (S1) glycoprotein gene. Phylogenetic analysis
revealed that the isolated strains were clustered into three distinct groups
within the variant II clade. Alignment of S1gene amino acid sequences showed
significant amino acid substitutions in relation to the currently used vaccinal strains which displays that new IBV variants which are
evolutionarily distant from vaccinal strains are still emerging within the
Egyptian chicken flocks and are mostly associated with acute tubular nephrosis
syndrome.
To Cite This Article: Magouz A, Abdo W,
Abdelsabour A, Elbestawy A and Desouky A,
2018. Molecular and histopathological
investigation of avian infectious bronchitis virus in the delta of egypt between
2016 and 2017. Pak Vet J, 38(3): 243-248. http://dx.doi.org/10.29261/pakvetj/2018.059