Detection of Mixed Infection of Avian Influenza
and Newcastle Disease Viruses in Chickens in Indonesia by
Immunopathologic Immunohistochemistry Double Staining
Raden Wasito*1, Hastari Wuryastuti2
and Bambang Sutrisno1
1Department
of Pathology, Faculty of Veterinary Medicine, Gadjah Mada
University, Yogyakarta, Indonesia; 2Department of
Veterinary Internal Medicine, Faculty of Veterinary Medicine, Gadjah
Mada University, Yogyakarta, Indonesia *Corresponding author: prof_wst@yahoo.com
Abstract
Avian influenza virus (AIV) and Newcastle disease
virus (NDV) are systemic disease that is highly infectious with high morbidity
and mortality rates, and highly detrimental to economic value in the domestic
and commercial poultry industries. Generally, in the field there is a mixed
infection of AIV and NDV, each of which is highly pathogenic. Clinical signs are
difficult to distinguish, so immuno-pathological immunohistochemistry (IHC)
double staining was developed with the ability to confirm the diagnosis of mixed
infection. In the present study, 20 chickens infected naturally with clinical
signs of torticollis and curled toe paralysis that had a mixture of AIV and NDV
infections and there are variations of tropism distribution of AIV and NDV in
tissues were used. The AIV and NDV were visualized by performing the double
staining technique DAB and Fast-Red with monoclonal antibody anti-hemagglutinin
AIV-mouse (AIVHA) and polyclonal antibody anti-hemagglutinin-neuraminidase
NDV-rabbit (NDVHN) proteins. AIV infected-lungs or brains had red coloration
(AIVHA) and NDV infected lungs or brains had brown coloration (NDVHN). The IHC
double staining technique is sensitive and accurate in the confirmation of the
diagnosis of a mixed infection to determine the difference between AIV and NDV.
To Cite This Article: Wasito R, Wuryastuti H and Sutrisno B, 2018.
Detection of mixed infection of Avian Influenza and Newcastle Disease Viruses
inchickens in Indonesia by immunopathologic immunohistochemistry doublé
staining. Pak Vet J, 38(4): 442-445. http://dx.doi.org/10.29261/pakvetj/2018.078