Matrine Inhibits Replication of Porcine
Reproductive and Respiratory Syndrome Virus (PRRSV) by Influencing
the Activation of Nsp9
Panpan Sun1,4, Na Sun1,
Zhirui Wang2, Jianhua Guo3, Yongming He4* and Hongquan Li1*
1College
of Animal Science and Veterinary Medicine, Shanxi Agricultural
University, Taigu, Shanxi 030801, P.R. China; 2Center for
Transplantation Sciences, Massachusetts General Hospital and Harvard
Medical School, Boston MA, USA; 3Department of Veterinary
Pathobiology, Schubot Exotic Bird Health Center, Texas A&M
University, College Station, TX 77843, USA; 4School
of Life Science and Engineering, Foshan university, Foshan,
Guangdong 528231, P.R. China *Corresponding author: livets@163.com; ymhe@fosu.edu.cn
Abstract
Matrine is the major active compound found in the
root of Chinese herbal medicine Sophora
flavescens and has a wide range of pharmacological efficacies. Our previous
studies showed that Matrine could inhibit the replication of porcine
reproductive and respiratory syndrome virus
(PRRSV) directly. However, the underlying
inhibition mechanism remains unknown. The main aim of this study was
to explore the inhibitory mechanism of Matrine in PRRSV replication
in vitro. The mRNA expression of PRRSV N gene was determined using
RT-qPCR. The expression of pERK1/2 protein was evaluated using Western blot and
the effect of Matrine on PRRSV Nsp9 activity was studied using dual luciferase
reporter gene assay. The results showed that Matrine suppress the mRNA
transcription of PRRSV N gene in Marc-145 cells at 3 h and 24 h post PRRSV
infection and had no inhibitory effect on the ERK1/2 signal pathway activated by
PRRSV. The results of dual luciferase reporter gene assay showed that Matrine
suppressed the interaction of Nsp9 with promoter p107. Taken into account the
results above, our study demonstrated that Matrine inhibit the activity of Nsp9
to suppress the virus replication.
To Cite This Article:
Sun P, Sun N, Wang Z, Guo J and Li H, 2018.
Matrine Inhibits Replication of Porcine Reproductive and Respiratory Syndrome
Virus (PRRSV) by influencing the activation of Nsp9. Pak Vet J, 38(4): 359-364.
http://dx.doi.org/10.29261/pakvetj/2018.079