Efficacy of Lytic
Bacteriophages against Multidrug Resistant Salmonella enteritidis from Milk and Meat
Abu Baker Siddique1*, Zeeshan Nawaz1,
Muhammad Asif Zahoor1, Azhar Rafique2, Bilal
Aslam1, Nida Nazia1 and Riaz Hussain3
1Department
of Microbiology, Government College University, Faisalabad, 38000-
Pakistan
2Department of Zoology, Government College University,
Faisalabad, 38000- Pakistan
3University
College of Veterinary and Animal Sciences, The Islamia University of
Bahawalpur, 63100- Pakistan
*Corresponding author:
absiddique@gcuf.edu.pk
Abstract
Salmonellosis is major food borne illness caused
by Salmonellaenteritidis. The emerging multidrug resistance has forced the
scientists to investigate alternative control strategies including phage therapy
to combat salmonellosis. Therefore, the current study was carried out to isolate
multidrug resistance S. enteritidis
from raw milk (n=40) and meat (n=30) samples. A total of 10
S. enteritidis isolates were recovered
from raw milk whereas 8 isolates were from meat samples. These isolates were
confirmed by Polymerase Chain Reaction using
spvC gene specific primers yielding 571bp product. The anti-biogram
studies were carried out on all isolates using different antimicrobials. The
results showed 11 (61%) isolates were susceptible to most of the antibiotics
while 7 (39%) isolates were found resistant and hence declared as multidrug
resistant S. enteritidis. In the
second step, sewage water was processed for the isolation of bacteriophages and
was used for bacteriophages enrichment against a susceptible strain of
S. enteritidis. Both clear and turbid plaques were isolated and
enumerated. The results of bacteriophages isolation showed a total of 7
different bacteriophages isolated out of 10 processed samples. Lytic potential
was measured by co-cultivation of S.
enteritidis with isolated bacteriophages. The results showed the significant
lytic potential of isolated bacteriophages against multidrug resistant
S. enteritidis isolates from raw milk
and meat samples. Altogether, the current study highlighted the significance of
S. enteritidis as an important multi
drug resistant food borne pathogen, further the bacteriophages therapy could be
an alternative control remedy.
To Cite This Article:
Siddique AB, Nawaz Z, Zahoor MA, Rafique A, Aslam
B, Nazia N and Hussain R, 2018. Efficacy of lytic bacteriophages against multidrug
resistant Salmonella enteritidis from milk and meat. Pak Vet J, 38(4): 353-358. http://dx.doi.org/10.29261/pakvetj/2018.092