Virulence Repertoire and Antimicrobial Resistance Profile of Shiga
Toxin-Producing E.coli Isolated from Sheep and Goat Farms
from Al-buhayra Egypt
Mohamed Sabry Abd
Elraheam Elsayed1*, Ashraf Awad2, Reda
Tarabees1 and Asmaa Marzouk3
1Department of Bacteriology,
Mycology and Immunology, Faculty of Veterinary Medicine, University
of Sadat City, Minufyia, Egypt; 2Department of
Bacteriology, Mycology and Immunology, Faculty of Veterinary
Medicine Banha University, Mushtohor; 3Veterinary
Administration Badr City Al-Buhayra Governorate, Egypt *Corresponding author:
mohamed.sabry@vet.usc.edu.eg
Abstract
Shiga toxin-producing E.coli (STEC)
evokes a paramount concern from the public health point of view. Many reports
dealt with the characterization of STEC from large ruminants. This study aimed
to investigate the presence of STEC in sheep and goats, distribution ofstx1, stx2, eaeA,and hlyA genes encodingShiga toxins, intimin,
enterohemolysins, and the antimicrobial resistance index (MAR). A total of 170 samples collected from (diarrheic, apparently healthy, and
milk samples) from sheep, goats, and bedding (136, 27 and 7) respectively.E.coli was detected at a rate of 71
(41.7%) distributed as 62 (44%) and 9
(31%) from sheep and goat, respectively. The prevalent
serotypes were O111: H2, O26: H11, O103:H2, O55: H7, O86, O121: H7, O125: H21, and
O124.The frequency of stx1 gene was 13/15 (86.7%),
stx2 was 14/15 (93.3%),the eaeA gene was 8/15 (53.3%), and
hlyA gene was 10/15 (66.7%). The most effective antimicrobials were
Chloramphenicol, Doxycycline and Cephradine. It was clear that 6/15 (40%) of the
obtained serotypes exhibited MAR index ≤0.5 while 9/15 (60%) gave MAR index ˃0.5
with a significant difference between them (P˂0.05). Hence, genotyping and
antimicrobial resistance are pivotal epidemiological tools promoting felicitous
control strategies against STEC serotypes.
To Cite This Article:
Elsayed MSAE, Awad A,
Trabees R and Marzouk A, 2018.
Virulence repertoire and antimicrobial resistance profile of shiga
toxin-producing E.coli isolated from sheep and goat farms from Al-Buhayra
Egypt. Pak Vet J, 38(4): 429-433. http://dx.doi.org/10.29261/pakvetj/2018.082