Characterization of Bacteriocin like Inhibitory
Substances from Enterococcus
ratti MF183967
Ayesha Riaz1, Saleha Noureen1,
Muhammad Fiaz Qamar2, Iram Liaqat3, Muhammad
Arshad4 and Najma Arshad1*
1Department
of Zoology, University of the Punjab, Quaid-i-Azam Campus,
Lahore-54590, Pakistan
2University
of Veterinary and Animal Sciences, Jhang, Pakistan; 3Government College
University, Lahore
4University of the Education, Lower Mall Campus,
Lahore- Pakistan
*Corresponding author:
najmaarshad@gmail.com; najmaarshad.zool@pu.edu.pu
Abstract
This study was designed to characterize one
Lactic acid bacteria (LAB), Enterococcus
ratti showing admirable antimicrobial potential. Out of 54 LAB strains
isolated from herbivores, cell free supernatant (CFS) of one strain
E. ratti was active against wide range of pathogens including
Methicillin resistant Staphylococcus
aureus (MRSA), Bacillus cereus,Clostridium perfringens,
Listeria monocytogenes,
Escherichia coli,
Salmonella enteritidis,
Pseudomonas aeruginosa, Klebsiella
pneumoniae and Proteus mirabilis.
PCR amplification of enterocin genes indicated presence of
entL50 A and entP. Time
kill assay of foodborne pathogens in the presence of CFS further confirmed its
bactericidal property. The antimicrobial component was stable at a wide range of
pH (4-10) and temperature (4-100°C). The loss of activity with proteinase k and
pepsin treatment indicated its proteinaceous nature. SDS-PAGE analysis confirmed
production of short peptides of 20 and 30KDa Bioautography revealed 20kDa
fraction was active against MRSA. Scanning Electron Micrograph further indicated
that its bactericidal action involves damage to the cell wall. Our findings
suggest safe nature of E. ratti
MF183967 for consumer health.
To Cite This Article:
Riaz A, Noureen S, Qamar MF, Liaqat I, Arshad
M and Arshad N, 2019. Characterization of bacteriocin like inhibitory substances
from Enterococcus ratti MF183967. Pak
Vet J, 39(1): 1-6. http://dx.doi.org/10.29261/pakvetj/2018.093