Contagious ecthyma or Orf is a highly contagious; zoonotic disease and despite the application of some control strategies against it, remains prevalent worldwide. Numerous measures such as anti-viral gene therapies are frequently employed to control viral infections such as ORFV. One way to evaluate these measures is the cloning of the viral target sequence into suitable vectors for the preparation of cell lines expressing sub- genomic replicons of the virus. In this study a conserved sequence of ORFV- DNA Pol was synthesized and cloned in pCDH-CMV-MCS-EF1-cGFP-T2A-Puro lentiviral vector at the upstream of GFP gene. The validity of cloning was confirmed by restriction enzyme digestion and sequencing. Therefore, this recombinant plasmid will be available to produce lentiviral vectors carrying this gene and after infection of Eukaryotic cells with such lentiviral vectors the expression of target gene will be induced.

To Cite This Article: Mokhtari A, Saffar B, Dehkordi NJ, Arani MJH, 2019. Cloning of a conserved domain of ORFV- DNA polymerase in the pCDH-CMV-MCS-EF1-cGFP-T2A-Puro lentiviral plasmid. Pak Vet J, 39(2): 305-309. http://dx.doi.org/10.29261/pakvetj/2019.028