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Effect of Active Immunization Using Gonadotropin-Releasing Hormone 2-Multiple Antigen Peptide with Different Adjuvants on the Reproductive Functions of Male Rats
Wenyu Si1,2§, Rongfei Zhu1,2§, Tiezhu Kang1,2 Shichun Ji1,2, Zhiqiu Yao1,2, Jing Ye1,2, Weiguo Tian1,2, Ya Liu1,2* and Fugui Fang1,2*
1Anhui Provincial Key Laboratory of Genetic Resources Protection and Biological Breeding in Local Livestock and Poultry, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; 2Department of Animal Veterinary Science, College of Animal Sciences and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; *Corresponding author:;


This study was aimed to compare the effects of different adjuvants on male reproductive function in rats immunized using GnRH2-MAP. Male Sprague-Dawley rats were assigned to control, ISA 201 adjuvant and Gel 01 adjuvant groups along with GnRH2-MAP. The serum testosterone and antibody titers were measured every 2-week by ELISA, the testes were weighed and histologically analyzed. mRNA expression levels in the hypothalamus, pituitary, and testes were determined by RT-qPCR. Among the three groups, the ISA 201 adjuvant group exhibited the lowest testosterone concentration (P<0.05), the highest antibody titer (P<0.05), and testis atrophy. GnRH mRNA expression in the hypothalamus; GnRH-R, FSH-β, and LH-β expression in the pituitary; and FSH-R, LH-R, and 3β-HSD expression in the testes were significantly lower in this group (P<0.01). Additionally, several other genes showed significant differential expression in the ISA 201 adjuvant group than in the other two groups. It is concluded that the GnRH2-MAP vaccine emulsified with ISA 201 adjuvant is more effective than that administered with Gel 01 adjuvant.

To Cite This Article: Si W, Zhu R, Kang T, Ji S, Yao Z, Ye J, Tian W, Liu Y and Fang F, 2019. Effect of active immunization using gonadotropin-releasing hormone 2-multiple antigen peptide with different adjuvants on the reproductive functions of male rats. Pak Vet J, 39(2): 205-210.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)