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Antibiotic Resistance Profiles and Virulence Markers of Escherichia coli Strains Isolated from Diarrheal Lambs in Gansu and Qinghai, China

Xin Tuo1, Shengyi Wang1, Dongan Cui1, Fuhe Wang1, Hongsheng Wang2, Yongming Liu1 and Hui Wang1,*

1Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; 2College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
*Corresponding author:


The current research was done to verify the prevalence, the antimicrobial resistant strains and virulence profiles of Escherichia coli in sheep of western China. In total 167 lambs diarrhea samples were collected from Gansu and Qinghai province, China, during 2014-2017. A total of 103 E. coli isolates were subjected to verify their antimicrobial susceptibility and virulence genes. The data showed that the percentage of E. coli isolates was 61.68%. The main resistances were obtained for penicillin (97.09%), lincomycin (95.15%) and erythromycin (69.90%). The most common antimicrobial resistance gene was tetA (83.50%), tetB (68.93%), blaTEM (63.11%), blaSHV (56.31%) and aac (3)-I (78.64%). Overall, the frequently virulence genes were mdh, ipaH, eae and stx1. The most frequent combined virulence patterns were ipaH-eae (38.83%), ipaH-stx1 (37.86%), eae-stx1 (33.98%), ipaH-astA (23.30%), astA-eae (21.36%) and ipaH-eae-stx1 (26.21%). These results demonstrated pathogenic E. coli are widely distributed in sheep of western China and carrying various antimicrobial resistance genes and virulence genes.

To Cite This Article: Tuo X, Wang S, Cui D, Wang F, Wang H, Liu Y and Wang H, 2020. Antibiotic resistance profiles and virulence markers of escherichia coli strains isolated from diarrheal lambs in Gansu and Qinghai, China. Pak Vet J, 40(1): 123-126.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)