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Prevalence, Genetic Diversity and Antimicrobial Resistance of Proteus mirabilis Isolated from Dogs Hospitalized in Beijing

Peipei Han1, Liping Luo2, Muhammad Shahid2Mengyue Li2, Shuhong Wang2, Diego B. Nobrega3, Naveed Sabir2, Herman W. Barkema3, Bo Han2 and Jian Gao2,*

1School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, 750004, China; 2College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China; 3Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada T2N 4N1
*Corresponding author:


Proteus mirabilis is an opportunistic pathogen that causes diarrhea and urinary tract infections in humans and companion animals. Emergence of antimicrobial resistance in P. mirabilis increases risk of failure of antimicrobial therapy in companion animals. The current study determined prevalence, genetic diversity and phenotypic antimicrobial resistance of P. mirabilis isolated from feces of 35 dogs with diarrhea hospitalized in Beijing, China. Random amplified polymorphic DNA (RAPD), pulse-field gel electrophoresis (PFGE), PCR and antimicrobial disk diffusion were used to characterize these isolates. Prevalence of P. mirabilis in feces from hospitalized dogs was 28%. RAPD and PFGE demonstrated a great diversity of isolates. Thirteen (37%) of 35 isolates produced extended-spectrum beta-lactamases (ESBLs), with blaTEM being detected in all ESBLs-producing isolates. All isolates were susceptible to imipenem, cefoxitin and cefotaxime/clavulanic. rmtB (with 51% prevalence rate) was the only aminoglycoside 16S rRNA methylase resistance gene identified. Among plasmid-mediated quinolones resistance genes, qnrB (14%) and aac(6’)-Ib (26%) were detected. In conclusion, P. mirabilis isolates from feces of dogs with diarrhea in China had great genetic diversity and a high prevalence produced ESBLs. Consequently, antimicrobial stewardship programs should also target companion animals to reduce emergence of antimicrobial resistance.

To Cite This Article: Han P, Luo L, Shahid MLi M, Wang S, Nobrega DB, Sabir N, Barkema HW, Han B and Gao J, 2020. Prevalence, genetic diversity and antimicrobial resistance of proteus mirabilis isolated from dogs hospitalized in Beijing. Pak Vet J, 40(1): 61-66.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)