previous page   Pak Vet J, 2020, 40(2): 187-194   next page
Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens

Hammad Qamar1, Aoyun Li1, Zhibo Zeng1, Yaping Wang1, Khalid Mehmood1,3, Muhammad Waqas1,4, Mudassar Iqbal1,3, Jialu Zhang1 and Jiakui Li1,2*

1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China; 2College of Animals Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Linzhi, Tibet 860000, PR China; 3University College of Veterinary & Animal Sciences, Islamia University of Bahawalpur-63100, Punjab, Pakistan; 4Faculty of Veterinary & Animal Sciences, University of the Poonch, Rawalakot, District Poonch 12350, Azad Jammu & Kashmir, Pakistan
*Corresponding author:


Among avian metabolic disorders, Tibial dyschondroplasia (TD) has a wide prevalence worldwide in chickens that are identified by non-mineralization and avascularization of the growth plate (GP). Grape seed extract (GSE) reduces the inflammation and oxidative stress by scavenging free oxygen specie and has some anti-cancer and angiogenic activity. This study was carried out to evaluate the role of GSE for the therapy of the chickens affected with TD. Arbor Acres broiler chicks (n=240) weighing 47±6g were fed starter feed till first 3 days and then portioned equally into three groups (Control, TD and GSE group; n=80 each). Thiram was incorporated in the feed of TD and GSE chicks until day 6 for inducing TD (50 mg/ kg of feed), whereas usual feed was offered to control group. All the birds in TD and GSE group showed the symptoms of TD on day 7 and GSE group was given GSE (50 mg/kg/day) intraperitoneally till the termination of the experiment. Chickens fed with thiram-feed exhibited high TD score and GP measurement. After the GSE treatment, the TD score dropped remarkably and, inflammation and TD lesions reduced. Moreover, body weight gain and liver weight were increased in comparison with TD chicks after application of GSE. GSE supplementation alleviated the adverse effects of thiram and increase in angiogenesis was observed. In conclusion, GSE recovered the TD affected broilers through downregulating the HIF-1α, Hsp90 and CA2 expressions.

To Cite This Article: Qamar H, Li A, Zeng Z, Wang Y, Mehmood K, Waqas M, Iqbal M, Zhang J and Li J, 2020. Effect of grape seed extract on tibial dyschondroplasia incidence, liver weight, and tibial angiogenesis in chickens. Pak Vet J, 40(2): 187-194.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)