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Efficacy of Kaolin and Bentonite Clay to Reduce Aflatoxin M1 Content in Contaminated Milk and Effects on Milk Quality

Amira I Moussa1*, Azza MK Sobeih2, Ibrahim I Al-Hawary2,3, Walaa M Elkassas1 and Radwa Barakat4,5*

1Food Hygiene Department, Animal Health Research Institute, Kafr El-Sheikh Branch, Kafr El-Sheikh, 33611, Egypt
2Food Hygiene Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
3Fish processing and Biotechnology, Dean of Faculty of Aquatic and Fisheries Science, Kafrelsheikh University, Egypt
4Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 61802, USA; 5Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Benha University, Qalyubia 13518, Egypt
*Corresponding author:;;


Mycotoxins contamination in milk products represents a major problem for milk industries. Aflatoxin M1 (AFM1) is very stable and resists any heat treatments as pasteurization and sterilization methods. The aim of this study was to determine the level of aflatoxin M1 in fifty raw milk samples collected from different dairy shops in Kafr El-Sheikh governorate in Egypt. We also evaluated the efficacy of detoxification methods of AFM1 in milk by using natural clay as Kaolin and Ca-bentonite. The milk survey study revealed that AFM1 was detected in all the examined raw milk samples with mean value of 10.7±0.89 ppb, which exceeded the Egyptian standard and European Union limit in raw milk. Then we artificially contaminated raw milk samples with AFM1 standard and then added three different concentrations of Kaolin and Ca- bentonite (5gm, 10gm, 20 gm), separately. The obtained results showed a significant reduction and detoxification in AFM1 concentration by Kaolin and Ca- bentonite treatments comparing with non-treated milk. The percent AFM1 detoxification rate by using kaolin and Ca- bentonite clay was 86.1 to 93.3% and 93.7 to 97.7%, respectively without any changes in nutritional constituents of milk. Moreover, the Ca-bentonite clay revealed a tendency to the comprehensive removal of AFM1 by increasing the quantity of clay added to the tested milk. These results indicate efficacy and safe usage of kaolin and Ca-bentonite clay to detoxification and reduce the amount of AFM1 in raw milk and consequently; minimize its dangerous effect on the public health.

To Cite This Article: Moussa AI, Sobeih AMK, Al-Hawary II, Elkassas WM and Barakat R, 2020. Efficacy of kaolin and bentonite clay to reduce aflatoxin M1 content in contaminated milk and effects on milk quality. Pak Vet J, 40(2): 181-186.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)