previous page   Pak Vet J, 2020, 40(2): 271-273   next page
In vitro Experiments Revealed the Anthelmintic Potential of Herbal Complex against Haemonchus contortus

Muhammad Arfan Zaman1*, Warda Qamar1, Saima Yousaf3, Uzma Mehreen1, Zohaib Shahid1, Muhammad Kasib Khan2, Muhammad Fiaz Qamar1 and Muhammad Kamran4

1Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore (Jhang Campus)
2Department of Parasitology, Faculty of Veterinary Sciences, University of Agriculture Faisalabad-Pakistan
3Department of Zoology, Wildlife and Fisheries, Faculty of Sciences, University of Agriculture, Faisalabad-Pakistan
4Department of Microbiology, University of Arid Agriculture, Rawalpindi (Khushab Campus)
*Corresponding author:


In an era of drug resistance, new medicinal plants are being evaluated for control of parasites. In the present study, the anthelmintic activity of the extract of herbal complex (HC) composed of four herbs (Origanum vulgare, Cinnamomum verum, Rosmarinus officinalis and Capsicum annuum) was assessed against eggs and adult Haemonchus (H.) contortus. Egg hatch test (EHT) and adult motility assay (AMA) was performed to evaluate the anthelmintic potential of HC. The reference drug used in EHT was oxfendazole and for AMA two drugs i.e. oxfendazole (2.265 mg ml-1) and levamisole (3mg ml-1) were used. In EHT, LC50 values of HC and oxfendazole were 498 and 1.6 ppm, respectively. In AMA, 100% mortality of H. contortus was observed after 6 hr of treatment with HC (100 mg ml-1) where as two positive control groups could not kill all worms after this exposure time. These results indicated the anthelmintic potential of HC.

To Cite This Article: Zaman MA, Qamar W, Mehreen U, Shahid Z, Khan MK, Qamar MF and Yousaf S, 2020. In vitro experiments revealed the anthelmintic potential of herbal complex against Haemonchus contortus. Pak Vet J, 40(2): 271-273.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)