Muhammad Arfan Zaman^1*, Warda Qamar¹, Saima Yousaf³, Uzma Mehreen¹, Zohaib Shahid¹, Muhammad Kasib Khan², Muhammad Fiaz Qamar¹ and Muhammad Kamran⁴

In an era of drug resistance, new medicinal plants are being evaluated for control of parasites. In the present study, the anthelmintic activity of the extract of herbal complex (HC) composed of four herbs (Origanum vulgare, Cinnamomum verum, Rosmarinus officinalis and Capsicum annuum) was assessed against eggs and adult Haemonchus (H.) contortus. Egg hatch test (EHT) and adult motility assay (AMA) was performed to evaluate the anthelmintic potential of HC. The reference drug used in EHT was oxfendazole and for AMA two drugs i.e. oxfendazole (2.265 mg ml^-1) and levamisole (3mg ml^-1) were used. In EHT, LC₅₀ values of HC and oxfendazole were 498 and 1.6 ppm, respectively. In AMA, 100% mortality of H. contortus was observed after 6 hr of treatment with HC (100 mg ml^-1) where as two positive control groups could not kill all worms after this exposure time. These results indicated the anthelmintic potential of HC.

To Cite This Article: Zaman MA, Qamar W, Mehreen U, Shahid Z, Khan MK, Qamar MF and Yousaf S, 2020. In vitro experiments revealed the anthelmintic potential of herbal complex against Haemonchus contortus. Pak Vet J, 40(2): 271-273. http://dx.doi.org/10.29261/pakvetj/2019.128