|
|
|
|
|
Pak Vet J,
2020,
40(3):
365-369
 |
|
Basic Analysis of Glycolysis in Cardiac Tissue in
Broiler Chickens Presenting with Ascites Syndrome |
|
Yaming Ge1§,
Yuan Li3§, Lingli Chen1, Haoyun Li2,
Han He2, Nan Huang2, Wenkui Wang2,
Hongmei Ning1* and Xiaowen Wang3* |
|
1College
of Animal Science and Veterinary Medicine, Henan Institute of
Science and Technology, Xinxiang, 453003, China; 2College
of Animal Science and Veterinary Medicine, Shanxi Agriculture
University, Taigu, 030801, China
3College
of Food Science and Engineering, Shanxi Agriculture University,
Taigu, 030801, China
*Corresponding author:
pangpangning@126.com |
|
Abstract
Ascites
syndrome (AS)
is one of the
most
noticeable
metabolic disorders in broiler chickens. In the present study, histopathology, immunohistochemistry, quantitative
real-time PCR and Western blot were
performed to investigate the influence of metabolic functions on the development
of AS. Pathological examination of cardiac tissues of AS broilers revealed
loosely arranged
myocardial fibers,
blurred
plasma membrane
boundaries, interstitial edema, and
congestion.
Levels of glycolysis
were analyzed in
cardiac tissue samples
based on the expression of
major
rate-limiting enzymes in the related pathway. The
results showed that the mRNA levels of PFK and PKM were significantly increased
2.19-fold and 3.82-fold vs. the control group in AS group (P<0.05),
respectively. IHC results showed increased protein levels of PFK1, p-PFK2 and
PKM2, which were in accordance with the changes in the levels of mRNA. Then, the
Western blot analysis demonstrated that the expression percentage of PFK1,
p-PFK2 and p-PKM2 were increased by 48.71, 45.41 and 67.14% than healthy
broilers (P<0.01). The results indicated that the
acidic intermediate products induced by the activation of glycolytic
pathway might trigger
fluid accumulation in the abdominal cavity of
broilers with AS.
To Cite This Article:
Ge Y, Li Y, Chen L, Li
H, He H, Huang N, Wang W, Ning H and Wang X,
2020. Basic analysis of glycolysis in cardiac tissue in broiler chickens
presenting with ascites syndrome. Pak Vet J, 40(3): 365-369.
http://dx.doi.org/10.29261/pakvetj/2020.060
|
|
|
|
|
|
|
ISSN 0253-8318 (Print) ISSN 2074-7764 (Online)
|