Tendinopathy or tendon degeneration in equine is an illness caused by inflammation or injury. Being a difficult tissue to repair, tendon damage can cause physical disabilities. Successful tendon regeneration requires an appropriate in-vitro model for tissue injury. Current study was planned to compare the effect of two inflammatory mediators: prostaglandinE2 (PGE2) and hydrogen peroxide (H₂O₂) on equine tendon fibroblasts. Tendon fibroblast cultures were subjected to two different doses of PGE2 (100ng/ml and 200ng/ml) and H₂O₂ (500µM and 1mM). Compared with untreated tendon fibroblasts, all doses of PGE2 and H₂O₂ reduced cell viability markedly. Viability was assessed by trypan blue staining method and crystal violet staining. Lactate dehydrogenase (LDH) release was measured to assess injury and cell proliferation by MTT assay. In comparison of dose, 100ng/ml of PGE2 and 500µM of H₂O₂ showed optimal results, whereas, higher dose exhibited highly reduced cell viability and increased cytotoxicity, which may cause difficulty in studying tendon injury in-vitro model. These findings suggest that both PGE2 and H₂O₂ have equal potential to cause tendon fibroblasts injury. Therefore, H₂O₂ can be used as cost-effective in-vitro model for tendon degeneration studies.

To Cite This Article: Shehzadi S, Javed M, Awan SJ, Nadeem A and Yaqub T, 2020. Developing a novel, efficient and cost effective tissue injury model (in-vitro) on equine tendon fibroblasts. Pak Vet J, 40(3): 360-364. http://dx.doi.org/10.29261/pakvetj/2020.058