G Bazekin*, I Gatiyatullin, E Skovorodin, A Sharipov and I Dolinin

The paper describes the glycyrrhizinic acid effect on rats having an experimental myocardosis using histological and immunohistochemical methods. The studies were performed on 30 sexually mature white Wistar rats of both sexes weighing 280-350 g. Dystrophic and necrotic processes were induced using adrenaline in rats' myocardium. The first experimental group received glycyrrhizinic acid at a dose of 50 mg/kg, together with drinking water for 14 days. The second experimental did not receive glycyrrhizinic acid. The third experimental group was a control group. 14 days after prophylactic use of glycyrrhizinic acid, the first and the second experimental groups were exposed to a single administration of epinephrine at a dose of 4 mg/kg. In the second experimental group, rats received glycyrrhizinic acid orally together with water once a day within 14 days, and then 0.1% adrenaline hydrochloride solution was injected subcutaneously. Morphological changes were studied in the responsive areas of the myocardium. There were found areas of extensive focal cardiosclerosis formed at the site of dead cardiomyoscytes, perivascular sclerosis, and thicker vascular wall due to cells' smooth muscle hyperplasia 30 days after a single injection of adrenaline in the rat myocardium. Glycyrrhizinic acid in rat myocardium stimulates increased cell expression of a tissue inhibitor of metalloproteinase-2 which has an antiapoptotic effect. This promotes the growth and survival of cardiomyocytes, prevents gross myocardial scarring in the affected areas, which ensures the cardioprotective effect of glycyrrhizinic acid in the adrenaline model of myocardial damage.

To Cite This Article: Bazekin G, Gatiyatullin I, Skovorodin E, Sharipov A and Dolinin I, 2020. Morphofunctional assessment of the glycyrrhizinic acid effect on myocardium of rats under adrenaline loading. Pak Vet J, 40(3): 289-294. http://dx.doi.org/10.29261/pakvetj/2020.024