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Metagenomics of Mosquito-borne Flaviviruses in Various Geoclimatic Districts of Punjab, Pakistan
Kashaf Yaseen1, Muhammad Sohail Sajid2,3, Muhammad Imran Arshad1,3, Rizwan Aslam1, Sultan Ali1*, Muhammad Saqib4, Waseem Akram5, Haider Abbas6, Abdullah Malik2, Umar Khalid1 and Faiz Ahmad Raza7
1Institute of Microbiology, University of Agriculture, Faisalabad-38040, Pakistan; 2Department of Parasitology, University of Agriculture, Faisalabad-38040, Pakistan; 3One Health Laboratory, Center for Advanced Studies in Agriculture and Food Security (CAS-AFS), University of Agriculture, Faisalabad-38040, Pakistan; 4Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad-38040, Pakistan; 5Department of Entomology, University of Agriculture, Faisalabad-38040, Pakistan, Head of Research and Development Program on Dengue, Chief Minister’s Secretariat, Lahore, Pakistan; 6KBCMA College of Veterinary & Animal Sciences (CVAS), Narowal, Sub-campus of University of Veterinary & Animal Sciences, Lahore, Pakistan; 7Senior Research Officer/ Centre in charge, Affiliation Pakistan Medical Research Council, PMRC Research Centre, Punjab Medical College, Faisalabad
*Corresponding author:


Mosquitoes are highly active vectors capable of transmitting various pathogenic and infectious diseases to humans and animals. The present study was designed to identify the major species of mosquitoes and mosquito-borne flaviviruses (Saint Louis Encephalitis, West Nile and Dengue Viruses) prevalent in three districts of Punjab representing the three agro-geoclimatic zones viz; Multan, Chakwal and Jhang. The collected mosquitoes were stereoscopically identified to confirm the species and sex of the mosquitoes. Stereoscopic identification confirmed that female mosquitoes were 4334 out of 10675 (40.6%), 2242 out of 7296 (30%) and 2040 out of 6450 (31.6%) from districts Chakwal, Jhang and Multan, respectively. It was concluded that Culex species were present in abundance (73.2%) as compared to Aedes species (26.7%) in the selected study districts. The results of multiplex RT-PCR depicted that Dengue, West Nile, Japanese encephalitis and Saint Louis encephalitis viruses were prevalent in the mosquitoes of all three selected districts. However, the prevalence of mosquito-borne viruses insignificantly varied (P>0.05) among the three districts. This study has provided a better understanding of the prevailing mosquito species and mosquito-borne viruses in the study districts that can help to devise appropriate control measures.

To Cite This Article: Yaseen K, Sajid MS, Arshad MI, Aslam R, Ali S, Saqib M, Akram W, Abbas H, Malik A, Khalid U and Raza FA, 2020. Metagenomics of mosquito-borne flaviviruses in various geoclimatic districts of Punjab, Pakistan. Pak Vet J, 40(4): 407-412.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)