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Identification and Molecular Characterization of Eimeria tenella based on EtMic5 Gene in Pakistan
Sayyed Raza Ali Shahid1, Muhammad Ali Shah1*, Aayesha Riaz1, Arshad Mahmood Malik2, Murtaz ul Hasan1, Li Xiangrui3 Wasim Babar4 and Syed Kamran Ali Shahid5
1Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS Arid Agriculture University Rawalpindi, Pakistan; 2Department of Economics, PMAS Arid Agriculture University Rawalpindi, Pakistan;
3Department of Preventive Medicine, Nanjing Agriculture University, Nanjing PR China; 4Department of Parasitology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan; 5Faculty of Veterinary and Animal Sciences, University of Poonch, Rawalakot, Pakistan.
*Corresponding author:


The aim of this study was to investigate the molecular characterization and phylogenetic analysis of microneme gene 5 of Eimeria tenella (EtMic5) from Pakistan to confirm its evolutionary relationship among different Eimeria species. Birds were reared and infected with Eimeria tenella oocysts. Postmortem of birds revealed the presence of lesions within intestinal caeca. Oocysts were collected, sporulated and used for RNA extraction. RNA was converted to cDNA and analyzed for EtMic5 gene using polymerase chain reaction (PCR). PCR products were confirmed through gel electrophoresis and the samples positive for EtMic5 gene were cleared using PCR cleanup process. EtMic5 gene was partially sequenced from Macrogen® laboratory Korea. Phylogenetic analysis revealed that the sequence is similar to all those previously reported in other parts of the world. The nucleotide sequence was deposited in GenBank and the assigned accession number is MT684461. The outcomes of this investigation indicate the presence of high frequency of Eimeria tenella infection in Pakistan.

To Cite This Article: Shahid SRA, Shah MA, Riaz A, Malik AM, Hasan MU, Xiangrui L, Babar W and Shahid SKA, 2020. Identification and molecular characterization of Eimeria tenella based on EtMic5 gene in Pakistan. Pak Vet J, 40(4): 443-448.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)