PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2021, 41(2): 209-214

Evaluation of Antibacterial Potential of Raw Turmeric, Nano-Turmeric, and NSAIDs against Multiple Drug Resistant Staphylococcus aureus and E. coli Isolated from Animal Wounds

Iqra Sarwar¹, Ambreen Ashar¹, Ashar Mahfooz^1*, Amjad Islam Aqib², Muhammad Ijaz Saleem¹, Asif Ali Butt³, Zeeshan Ahmed Bhutta¹,Muhammad Shoaib⁴, Muhammad Fakhar-e-Alam Kulyar¹ and Athar Ilyas⁵

¹Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, 38000, Pakistan; ²Department of Medicine, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, 63100, Pakistan; ³Riphah International University, Faisalabad, 44000, Pakistan; ⁴Institute of Microbiology, University of Agriculture, Faisalabad, 38000, Pakistan; ⁵Department of Theriogenology, University of Agriculture, Faisalabad, 38000, Pakistan
*Corresponding author: drasharcms@gmail.com

Abstract

Wound healing gets difficult due to antibiotic resistance and requires prolong therapy. Current study was planned to check antibacterial potential of raw turmeric, nano-turmeric, and Non-steroidal anti-inflammatory drugs against multiple drug resistant (MDR) Staphylococcus aureus and E. coli isolated from animal wounds. Wound samples (n=150) were collected from different animals. Isolation and confirmation of S. aureus and E. coli was done by microbiological techniques and biochemical tests. Confirmed isolates were subjected to number of antibiotics for MDR S. aureus and E. coli. Drug modulation of MDR isolates were done by NT, RT, and NSAIDs in combination with antibiotics using well diffusion method. The data were analyzed by non-probability testing at 5% probability using SPSS statistical computer program. Study found 20.7 and 28.7% of MDR E. coli and S. aureus from animal wounds, respectively. MDR S. aureus were found 27.40, 33.30, and 100% sensitive while MDR E. coli were found 12.50, 62.50, and 75.00% against oxytetracycline, chloramphenicol, and trimethoprim-sulphamethoxozole, respectively. Nano curcumin showed higher antibacterial activity against MDR S. aureus and E. coli in comparison with raw curcumin. NSAIDs in combination with antibiotic also showed synergistic effect in inhibition of bacterial growth. Among the assumed risk factors external parasites, body condition and antibiotic use showed significant association. The study found higher prevalence of MDR S. aureus and E. coli from wounds with significant association of assumed risk factors, along with promising antibacterial effects of nano curcumin, raw curcumin, and NSAIDs in combination with antibiotics.

To Cite This Article: Sarwar I, Ashar A, Mahfooz A, Aqib AI, Saleem MI, Butt AA, Bhutta ZA,Shoaib M, Kulyar MFA and Ilyas A, 2021. Evaluation of antibacterial potential of raw turmeric, nano-turmeric, and NSAIDs against multiple drug resistant Staphylococcus aureus and E. coli isolated from animal wounds. Pak Vet J, 41(2): 209-214. http://dx.doi.org/10.29261/pakvetj/2021.014

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)