Designing of Multiepitope-based Subunit Vaccine
(MESV) against Prevalent Serotype of Foot and Mouth Disease Virus
(FMDV) using Immunoinformatics Approach
Rimsha Riaz1, Muhammad Sarwar Khan1*,
Faiz Ahmad Joyia1 and Muhammad Anjum Zia2
1Centre
of Agricultural Biochemistry and Biotechnology (CABB), University of
Agriculture Faisalabad, Pakistan
2Department of Biochemistry, University of Agriculture
Faisalabad, Pakistan
*Corresponding author:
sarwarkhan_40@hotmail.com
Abstract
Foot-and-Mouth Disease (FMD) is causing 100% morbidity in adult animals with a
high mortality rate in young animals due to cardiomyopathy. The disease-causing
virus (FMDV) has been classified into seven serotypes and several topotypes.
Amongst serotypes, the serotype O is predominantly present in Pakistan. A
vaccine that should be safer and appears as an alternative to conventional
vaccines that have failed to control the disease is required. Keeping in view, a
multiepitope-based subunit vaccine (MESV) is designed using computational
biology-based approaches. The sequence and structural characterization of capsid
proteins and 3Cpro of O PanAsia-II sub-lineage are carried out for
screening the antigenic proteins. Further, the B- and T-cell epitopes are
selected based on toxicity, antigenicity, allergenicity, conservancy, and
topology. The Cytotoxic T lymphocyte (CTL) epitopes are confirmed through
molecular interactions with selected bovine alleles. The designed MESV is found
stable, hydrophilic, antigenic, non-allergen, exposed extracellularly,
non-toxic, and soluble. It will stimulate the protective immune response against
the viral challenge as it has shown a strong binding affinity with the TLR4.
These results suggest that the developed MESV will prove to be a potential
candidate vaccine in controlling the disease against serotype O. For expression
and accumulation of stable protein the sequence that encodes the multiepitope-based
subunit vaccine is codon-optimized following the codon preference of E. coli
and is cloned in pET-28a (+).
To Cite This Article:
Riaz R, Khan MS, Joyia FA and Zia MA, 2021.
Designing of Multiepitope-based Subunit Vaccine (MESV) against prevalent
serotype of Foot and Mouth Disease Virus (FMDV) using immunoinformatics
approach. Pak Vet J, 41(3): 400-408.
http://dx.doi.org/10.29261/pakvetj/2021.033