Silver Nanoparticles:
Green Synthesis, Characterization, Blood Compatibility and
Protoscolicidal Efficacy against Echinococcus granulosus
Parwin Jalal Jalil1,
Bushra Hussain Shnawa1,2,*and Samir Mustafa Hamad2
1Biology
Department, Science Faculty, Soran University, Kurdistan-Iraq
2Scientific
Research Centre, Soran University, Kurdistan- Iraq
*Corresponding author:
bushra.shnawa@soran.edu.iq
Abstract
Spillage of protoscoleces within hydatid fluid
during surgery for hydatid cyst is the main reason for its
recurrence. Therefore, to inactivate the protoscoleces, various scolicidal
substances have been tested. However, novel and more efficient agents are needed
owing to several associated complications. This study focused on the effects of
green synthetic Silver Nanoparticles (AgNPs) from Zizyphus spina- christi
leaves on Echinococcusgranulosus protoscoleces. Also, to
evaluate the blood compatibility of Ag
NPs.The Ag NPs were identified by ultraviolet-visible
(UV-Visible) spectrophotometer, X-ray diffraction (XRD), Scanning electron
microscopy imaging, and Energy-dispersive X-ray spectroscopy (EDX). Hydatid
fluid was aspirated aseptically from cysts of infected sheep liver. The
protoscoleces were exposed to Ag NPs at several concentrations. Also, scanning
electron microscopy for ultrastructural changes and in vitro erythrocytes
lysis was performed. The Ag NPs were spherical; the particles' size reached
50 nm, and presented a surface plasmon peak around 460 nm. The current study's
findings indicated the powerful in vitro scolicidal efficacy of the green
biosynthesized AgNPs. Several morphological alterations were observed on the
protoscoleces by optical and scanning electron microscopy. Lysis of RBCs at
different doses of Ag NPs was significantly (P≤0.05) less than the positive
control value, thus proposing its biocompatibility. This work suggests that
chemicals like polyphenols present in the extract of Z. spina- christi
act as reducing and stabilizers agents to create Ag NPs Nevertheless, further
investigations are needed to investigate the Ag NPs scolicidial effects in
animal models.
To Cite This Article:
Jalil PJ, Shnawa BH,
and Hamad SM, 2021.
Silver nanoparticles: green synthesis, characterization, blood compatibility,
and protoscolicidal efficacy against Echinococcus granulosus. Pak
Vet J, 41(3): 393-399. http://dx.doi.org/10.29261/pakvetj/2021.039