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Possible Role of Kisspeptin in Regulation of Motility Spectrum of Buffalo Bull Spermatozoa: A Preliminary Study
Lubna Khan1,2,3*, Shazia Shamas1,2, Hussain Ahmed3,4, Hira Zubair1, S Murtaza Hassan Andrabi3, Riffat Bano1, Bakht Tarin Khan4 and Muhammad Shahab1*

1Laboratory of Reproductive Neuroendocrinology, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320 Islamabad, Pakistan; 2Department of Zoology, University of Gujrat, Gujrat, Pakistan;3Animal Reproduction Laboratory, Animal Sciences Institute, National Agriculture Research Centre, Islamabad, Pakistan; 4Department of Zoology, University of Buner, Khyber Pakhtunkhwa, Pakistan
*Corresponding author:;


This study was designed to determine possible effects of human kisspeptin-10 (KP-10) on motility spectrum of buffalo bull spermatozoa. Semen was collected from three adult Nili-Ravi buffalo bulls twice a week for three-weeks, split into five aliquots and diluted with different doses (0, 0.2, 2.0, 20.0 and 40.0 µM) of KP-10 in 0.1% BSA-PBS. The aliquots were incubated for 2, 6, 12 and 24 min at 37°C and evaluated through Computer-Assisted Sperm Analysis (CASA) system for sperm motility parameters. Ethanol fixed smears were processed for immuno-cytochemical detection of KP. Results showed that KP-10 exposure (mostly at 20.0 and 40.0 µM) for different incubation time-points significantly (P<0.05) improved CASA sperm motilities, velocities, and rapid subpopulation than control. Furthermore, buffalo bull spermatozoa showed KP-10 expression in their tail region. This study identified the stimulatory effect of human KP-10 on buffalo bull sperm motility parameters during in vitro incubation. Thus, human KP-10 can be potentially used to enhance quality of buffalo bull sperm during incubation at 37°C in vitro.

To Cite This Article: Khan L, Shamas S, Ahmed H, Andrabi SMH, Zubair H, Bano R, Khan BT and Shahab M, 2021. Possible role of Kisspeptin in regulation of motility spectrum of buffalo bull spermatozoa: A preliminary study. Pak Vet J, 41(4): 579-582.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)