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An Economical Non-Antibiotic Alternative to Antibiotic Therapy for Subclinical Mastitis in Cows
Misbah Ijaz1, Asad Manzoor1*, Muhammad Tahir Mohy-ud-Din1, Faiza Hassan2, Zia Mohy-ud-Din3, Muhammad Ans4, Muhammad Ijaz Saleem1, Hamza Hassan Khan3 and Fakhra Khanum5

1Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad
2Institute of Physiology and Pharmacology, Faculty of Veterinary Science, University of Agriculture, Faisalabad
3Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture, Faisalabad
4Department of Pharmacology, Lahore Medical & Dental College, Lahore, Pakistan; 5Department of Nutrition and Dietetics, The University of Faisalabad, Faisalabad, Pakistan
*Corresponding author:


The economic importance of mastitis and antibiotic resistance is dictating to search non-antibiotic alternatives for the therapy. Trisodium citrate (TSC) being buffer system of the glandular tissue and, vitamin C (Vit. C), zinc and copper being important ingredients required for functioning of immune system fancy chances for a suitable alternative mastitis therapy. The current study was planned to evaluate therapeutic efficacy and cost effectiveness of these ingredients in subclinical mastitis. For this purpose, 40 sub-clinically mastitis cows were randomly divided into 2 equal groups. Group T1 was treated orally with TSC, Vit. C, ZnSo4 and CuSo4, while group T2 was treated with standard antibiotic therapy.  Milk pH significantly (P<0.05) differed between the two treatments till day 7th post-initiation of treatment when T1 restored the pH values within normal range earlier than T2. A non-significant (P>0.05) difference was observed in milk pH, fat, lactose, proteins, TS, SNFs, somatic cell counts and restoration of milk yield between the two treatments indicting comparable efficacy. A statistically significant (P<0.05) difference was observed in serum Cu and Zn levels indicating that the supplementation of Cu and Zn led to higher serum values in animals of T1. The use of non-antibiotic oral formulations as mastitis therapy resulted in a net profit of Rs. 457/animal/day.  The oral non-antibiotic antibacterial formulation is a therapeutically and economically suitable alternative to rational antibiotic-based therapy to treat subclinical mastitis in dairy cows.

To Cite This Article: Ijaz M, Manzoor A, Mohy-ud-Din MT, Hassan F, Mohy-ud-Din Z, Ans M, Saleem MI, Khan HH and Khanum F, 2021. An economical non-antibiotic alternative to antibiotic therapy for subclinical mastitis in cows. Pak Vet J, 41(4): 475-480.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)