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Evaluation of the Inhibitory Effects of Coumermycin A1 on the Growth of Theileria and Babesia Parasites in vitro and in vivo
Mahmoud AbouLaila1, 2, Amer Ragheb AbdEl-Aziz 3, Soad Menshawy2, Naoaki Yokoyama1, Ikuo Igarashi1, Mohammad Al-Wabel4 and Mosaab Omar4,5*

1National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan; 2Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Elbehera, Egypt; 3Department of Parasitology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt; 4Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, 51452 Qassim, Saudi Arabia; 5Department of Parasitology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Qena, Egypt
*Corresponding author:


Coumermycin A1, a coumarin antibiotic, has anticancer, antibacterial, antiviral, and antimalarial activities. We aimed to evaluate the anti-thielerial and anti-babesial activity of coumermycin A1 in mice in vivo. Coumermycin A1 efficacy was determined by the transcription of DNA gyrase, a type II DNA topoisomerase using reverse transcriptase-polymerase chain reaction (RT-PCR) transcription. Coumermycin A1 significantly inhibited the development of preliminary parasitemia (1%). Theileria equi and the Babesia species B. bigemina, B. bovis, and B. caballi were observed with IC50 values of 80, 70, 57, and 65 nM, respectively. Their development was remarkably inhibited at observed concentrations of 10, 25, 50, and 100µM for the studied organisms T. equi, and the Babesia species B. caballi, B. bovis and B. bigemina, respectively. In the subsequent viability test, parasite re-growth was suppressed at 100µM for B. bigemina and B. bovis and at 50 µM for B. caballi and T. equi. Coumermycin A1 Treatment of B. bovis cultures with Coumermycin A1 completely suppressed the transcription of the DNA gyrase subunits B and A genes. In BALB/c mice, the development of Babesia microti was inhibited by 70.73% using 5 mg/kg of Coumermycin A1.

To Cite This Article: AbouLaila M, AbdEl-Aziz AR, Menshawy S, Yokoyama N, Igarashi I, Al-Wabel M and Omar M, 2021. Evaluation of the inhibitory effects of coumermycin A1 on the growth of Theileria and Babesia parasites in vitro and in vivo. Pak Vet J, 41(4): 469-474.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)