Frequency and Antimicrobial Susceptibility of
Methicillin and Vancomycin-Resistant
Staphylococcus aureus from
Bovine Milk
Muhammad Umar Javed1, Muhammad Ijaz1*, Zahida Fatima2, Aftab Ahmad Anjum3, Amjad Islam
Aqib4, Muhammad Muddassir Ali5, Abdul Rehman6,
Arslan Ahmed1 and Awais Ghaffar1
1Department
of Veterinary Medicine, University of Veterinary and Animal
Sciences, Lahore, Pakistan
2Animal
Sciences Division, Pakistan Agricultural Research Council,
Islamabad, Pakistan
3Institute
of Microbiology, University of Veterinary and Animal Sciences,
Lahore, Pakistan
4Department
of Medicine, Cholistan University of Veterinary and Animal Sciences,
Bahawalpur, Pakistan
5Institute
of Biochemistry and Biotechnology, University of Veterinary and
Animal Sciences, Lahore, Pakistan
6Department
of Epidemiology and Public Heath, University of Veterinary and
Animal Sciences, Lahore,
Pakistan
*Corresponding author:
mijaz@uvas.edu.pk
Abstract
The increase in resistance pattern of
Staphylococcus aureus (S. aureus)
has been an emerging threat in therapeutic areas of the dairy industry
throughout the globe. The current study was conducted in bovines of district
Faisalabad, Pakistan to investigate the phenotypic prevalence of methicillin-resistant
(MRSA) and vancomycin-resistant S. aureus
(VRSA) in milk samples positive for subclinical mastitis. The study further
aimed to assess the associated risk factors and antimicrobial susceptibility
pattern against MRSA and VRSA isolates. A total of 385 milk samples (n=193
cattle; n=192 buffalo) collected and screened for subclinical mastitis by surf
field mastitis test (SFMT) were further subjected to standard microbiological
techniques for the isolation of S. aureus.
The positive isolates of S. aureus
were phenotypically evaluated for MRSA and VRSA by the disc diffusion method.
The study results revealed that out of 385 milk samples, 45.97% (177/385)
samples were found positive for subclinical mastitis on SFMT while 37.14%
(143/385) samples were confirmed for the presence of
S. aureus. Out of these S.
aureus isolates, MRSA and VRSA were confirmed in 17.48% (25/143) and 12.58%
(18/143) samples respectively. The
in-vitro trials of various antibiotics for MRSA and VRSA isolates
showed 100% resistance towards Cefoxitin
followed by 50% towards Gentamicin, Tylosin, and Trimethoprim + Sulfamethoxazole
then 25% to Oxytetracycline, and Fusidic acid while ciprofloxacin, moxifloxacin,
and linezolid were found sensitive against study isolates. The public health
importance of S. aureus and emerging
resistance against antibiotics like methicillin and vancomycin demands regular
monitoring of effective use of antimicrobial agents against the isolates of VRSA
and MRSA.
To Cite This Article:
Javed MU, Ijaz M, Fatima Z,Anjum AA, Aqib AI, Ali MM, Rehman A, Ahmed A and Ghaffar A, 2021.
Frequency and antimicrobial susceptibility of methicillin and vancomycin-resistant
Staphylococcus aureus from bovine milk.
Pak Vet J, 41(4): 463-468.
http://dx.doi.org/10.29261/pakvetj/2021.060