The current experimental study determined the lipid lowering effects of corn flour in albino mice. A total 180 adult albino mice having average body mass (30±9 g) were procured and kept in clean and aerated plastic cages. After 7 days of acclimatization, all the mice were randomly divided and kept in six equal groups consisting of groups A and B (untreated and treated control) and treated groups (C-F). Mice were fed diet containing cholesterol (400 mg/kg body weight) for induction of hyperlipidemia. All the groups were given atherogenic diet for 15 days except group A. Standard drug (Simvastatin) was given to control group @ 0.6mg/kg at day 15 till the completion of experiment. The mice were given corn flour in group C (1 gm/kg), D (2 gm/kg), E (4 gm/kg) and F (6 gm/kg) at day 15 up to day 60 of trial. Blood was collected for serum separation from each mice at days 0, 15, 30, 45, and 60 of trial. The results on serum biochemistry indicated that supplementation of different concentrations of corn flour significantly altered serum lipid profile (TL, TC, TG and LDL) in albino mice. The values of HDL were increased in mice of corn flour treated groups. Results showed that corn flour at 6 mg/kg body weight significantly lowered cholesterol levels in treated mice.