Determination of Apoptosis and Autophagy in
Bovine Ocular Squamous Cell Carcinomas by Immunohistochemistry
Ayfer Yildiz* and Emin Karakurt
Kafkas University, Faculty of Veterinary
Medicine, Department of Pathology, Kars, Turkey *Corresponding author:yildizayfer360@gmail.com
Abstract
Ocular squamous cell carcinoma (OSCC) is one of
the most common neoplasms in cattle in Kars province, Turkey. This study was
performed on 45 cases of OSCC sent by the Kafkas University Faculty of
Veterinary Medicine Research Hospital and private veterinary clinics to the
university pathology department between the years 2012 and 2021. The removed
tumor masses were macroscopically different in size and in hemorrhagic degree,
and their surfaces were smooth or cauliflower-like. The removed masses were
stained with hematoxylin–eosin for the histopathological examination,
which showed neoplastic formations
characterized by differentiation of atypical keratinocytes from the epidermis to
the dermis. The cases were divided into three groups according to the density
and number of tumor pearls, mitotic activity and their pleomorphism status: well
differentiated, moderately differentiated, and poorly differentiated. Caspase-3
and apoptosis-inducing factor (AIF) as apoptosis markers and protein light chain
3 (LC3B) as autophagy markers were stained by immunohistochemical methods to
determine the role of apoptosis and autophagy in OSCC cases. A statistically
significant difference was observed between the groups separated according to
the degree of differentiation in caspase-3 and LC3B immunostaining; however,
non-significant difference in AIF immunostaining was found among three groups.
The histopathological and immunohistochemical evaluations showed that caspase-dependent
apoptosis was more predominant than caspase-independent apoptosis in OSCC cases
of cattle, and autophagy and apoptosis might play a common role in tumor
aggressiveness and progression.
To Cite This Article:
Yildiz A, Karakurt E,
2022. Determination of apoptosis and autophagy in bovine ocular squamous cell
carcinomas by immunohistochemistry. Pak Vet J, 42(2): 147-152.
http://dx.doi.org/10.29261/pakvetj/2022.032