The organic and inorganic constituents present in cystic fluid play a crucial role in the physiology, metabolism and immune responses mediated by taeniids. We designed a comparative study on T. hydatigena and E. granulosus cysts based on biochemical profile. In the present study, we aspirated fluid from Taenia hydatigena (n=30) and Echinococcus granulosus (n=30) cysts recovered from slaughtered sheep and goats in the central abattoir of Faisalabad, Pakistan. The fluid obtained from each cyst was subjected to biochemical analysis to estimate the levels of liver enzymes, electrolytes and selected biomolecules (proteins, glucose, cholesterol, urea, and creatinine). Thereafter, the student’s t-test was used to determine significant differences between constituents of the cystic fluids obtained from both type of cysts. The difference was considered statistically significant at P<0.05. The levels of total protein and globulin differed significantly (P<0.05) and were found to be higher in T. hydatigena cystic fluid. A non-significant difference was observed for albumin. Similarly, higher concentrations of electrolytes (sodium, chloride and magnesium) were observed in T. hydatigena cystic fluid with statistically significant difference (P<0.05). Concerning liver enzymes, high levels (p>0.05) of ALP and LDH were observed in T. hydatigena and E. granulosus cystic fluid had significantly higher (P<0.05) level of AST. The levels of cholesterol, urea and creatinine were higher in E. granulosus. In contrast, glucose was higher in T. hydatigena. A significant difference (P<0.05) was observed for these four biomolecules. Overall, these results suggest high variations of investigated biomolecules, liver enzymes, and electrolytes composition between cystic fluids of E. granulosus and T. hydatigena. Further studies are now warranted to set the standards for the differential diagnosis of cysticercosis and cystic echinococcosis based on cystic fluid contents.