First Molecular Evidence of
Ehrlichia Infection: An Emerging Pathogen of Small Ruminants in
Pakistan
Muhammad Abdul Basit1, Muhammad Ijaz1,
Rao Zahid Abbas2, Jawaria Ali Khan1 and Kamran
Ashraf3
1Department
of Veterinary Medicine, University of Veterinary and Animal
Sciences, Lahore-Pakistan
2Department
of Parasitology,
University of Agriculture, Faisalabad-Pakistan
3Department
of Parasitology, University of Veterinary and Animal Sciences,
Lahore-Pakistan
*Corresponding author:
mijaz@uvas.edu.pk
Abstract
The emergence and inter-species dissemination of
new tick-borne pathogens have become a serious health hazard in domestic
animals. Ehrlichiosis caused by
Ehrlichia (E.)
ruminantium in small ruminants is becoming an emerging and prevailing issue
adversely affecting the health and production of sheep and goats. The current
study was designed to investigate the
Ehrlichia infection load in the sheep and goat population of South Punjab,
Pakistan. The phylogenetic analysis of local isolates along with risk factor
analysis and comparative hematological analysis was also conducted. A total of
192 blood samples consisting of sheep (n=96) and goat (n=96) were subjected to
microscopy and PCR. The study results revealed an overall prevalence of 04.69
and 7.81% based on microscopy and PCR respectively. A higher molecular
prevalence of Ehrlichia was observed
in goats (9.38%) as compared to sheep (6.25%). The phylogenetic tree constructed
by the maximum likelihood method exhibited the identity of study isolate with
E. ruminantium isolated from various countries. The risk factor analysis
showed a significant association of previous tick history, tick infestation,
presence of shrubs, and tick control strategies adaptation with the disease
occurrence. The hematological analysis observed a significant reduction in RBCs,
hemoglobin, and hematocrit in infected animals compared to healthy animals. The
study concludes that Ehrlichia
infection is an emerging issue of small ruminants. The hematological findings
can be helpful in early detection of disease in field conditions but molecular
confirmation by PCR is necessary for accurate diagnosis of
Ehrlichia infection while risk factor
analysis will provide the relevant information to develop control measures for
preventing the spread of disease.
To Cite This Article:
Basit MA, Ijaz M, Abbas RZ, Khan JA and Ashraf K,
2022. First molecular evidence of
Ehrlichia infection: An emerging
pathogen of small ruminants in Pakistan. Pak Vet J, 42(2): 208-214.
http://dx.doi.org/10.29261/pakvetj/2022.012