Determination of
Canine Parvovirus Variants in Puppies by Molecular and Phylogenetic
Analysis
Irmak DIK1*, Mustafa Emin OZ2, Oguzhan AVCI1 and Atilla
SIMSEK1
1Department
of Virology, Faculty of Veterinary Medicine, University of Selcuk,
Konya, Turkey 2Molecular Microbiology Laboratory, Veterinary Control Institute,
Konya, Turkey
*Corresponding author: irmakdik@selcuk.edu.tr
Abstract
Canine Parvovirus-2 (CPV-2) causes infectious
viral disease characterized by severe diarrhea, vomiting, and high mortality,
especially in young dogs. For routine laboratory diagnosis and molecular
characterization of CPV-2, PCR analysis and phylogenetic analysis have been
widely used methods in recent years. The aim of this study was to determine the
molecular diagnosis and characterization of CPV-2 variants in puppies (0-6
months) with diarrhea symptoms and no vaccination against CPV-2 in the Central
Anatolia region of Turkey. The phylogenetic analyses for VP2 gene were performed
from the stools of the puppies (n = 50), which were found to be CPV-2 positive
for the presence of nucleic acid by PCR, showing signs of gastroenteritis that
were owned and cared for in the shelter environment. The results of the
phylogenetic analysis, which included the study sequences of 4 each female and
male puppies, indicated that both CPV-2a (4/8, 50%) and CPV-2b (4/8, 50%)
variants were circulating in the study area. Molecular analyses also revealed
that CPV-2 variants did not cause co-infection in these puppies. In conclusion,
it can be stated that CPV-2a and CPV-2b variants continue to exist among the
owned and stray dogs in the shelter in the Central Anatolia Region. Therefore,
new vaccines should develop for successful fight against the infection and the
vaccines should be prepared against CPV-2
virus antigenic types in different regions.
To Cite This Article:
DIK I, OZ ME, AVCI O, SIMSEK A 2022.
Determination of canine parvovirus variants in
puppies by molecular and phylogenetic
analysis. Pak Vet J, 42(2): 271-275.
http://dx.doi.org/10.29261/pakvetj/2022.024