Nanoparticles of Kirinyuh (Chromolaena odorata (L.)
R.M.King & H.Rob.) Leaves Extract as a Candidate for Natural
Remedies Lowering Hypercholesterol: In Silico and In vivo Study
1Faculty
of Veterinary Medicine, Universitas Gadjah Mada, Sleman, Yogyakarta,
55281, Indonesia; 2Faculty of Mathematics and Natural
Science, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281,
Indonesia; 3Department of Pharmacology, Faculty of
Veterinary Medicine, Universitas Gadjah Mada, Sleman, Yogyakarta,
55281, Indonesia
*Corresponding author:
anggi.muhtar.p@mail.ugm.ac.id
Abstract
Hypercholesterolemia in recent years has become a serious problem because of its
cause of almost half of cases of ischemic heart disease. Alternative medicine
most people usually use also has a weakness, namely weak pharmacological
effects. This study aims to optimize the pharmacological effect of kirinyuh
leaves as an antihypercholesterolemic agent by making nanoparticles
preparations. This study used in silico and in vivo studies, the in silico study
was done to predict the potential of flavonoid, one of kirinyuh leaves’
compounds which can inhibit HMG-CoA reductase (HMGCR) enzyme, that have a role
of cholesterol making. In vivo study was done to hypercholesterolemia mouse
model (n=24) who were given of kirinyuh leaves extract
each with 100 mg (T1) and 200 mg/200 g body
weight (BW) (T2) dose, and kirinyuh leaves extract in nanoparticle
formulas each 1 mL (T3) and 2 mL/200 g BW (T4) every day for 2 weeks. Blood was
snipped at the end of treatment for lipid profile analysis. Molecular docking’s
result showed that flavonoid compounds can reduce hypercholesterolemia by
interacting with HMGCR enzymes thus the formation of cholesterol in the liver
can be inhibited, therefore T1-T4 are capable to improve blood lipid profile
significantly compared to control (P<0.05). The best result is shown in T4 that
gave 2 mL/200 g BW dose of nanoparticle formulation with the most reduction of
total cholesterol and lipid profile between all the treatment groups. It can be
concluded that nanoparticle formulation has better pharmacologist effect.
To Cite This Article:
Salsabil SS, Ardana VP, Larastiyasa RRPB, Pratiwi IW, Widianti RA, Pratama AM
2022. Nanoparticles of kirinyuh (Chromolaena odorata (L.) R.M.King
& H.Rob.) leaves extract as a candidate for natural remedies lowering
hypercholesterol: in silico and in vivo study.
Pak Vet J, 42(3): 397-403.
http://dx.doi.org/10.29261/pakvetj/2022.023